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在大米中识别和表达酸氨基转移蛋白基因的基因表达分析

Mengtian Pei¹, Xuze Xie¹, Baoyi Peng¹

¹State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Plants (Basel, Switzerland)

|June 10, 2023

概括

这项研究确定了30种大米的酸氨基转移蛋白 (PITPs). 一些OsPITPs通过甲基斯酸和酸通路参与了大米对Magnaporthe oryzae感染的天生的免疫力.

科学领域:

植物分子生物学植物分子生物学
植物病理学植物病理学
生物化学生物化学

背景情况:

酸氨基转移蛋白 (PITPs) 对各种植物生物功能至关重要.
米 (Oryza sativa) 中PITPs的特定作用在很大程度上仍然没有特征.

研究的目的:

为了识别和描述大米PITPs (OsPITPs).
调查OsPITPs在大米对Magnaporthe oryzae.天生的免疫力中的潜在作用.

主要方法:

对大米基因组进行生物信息分析,以确定PITP基因.
对基因结构,物理化学性质,保存域和亚细胞局部化的分析.
检查激素反应元素 (甲基斯蒙酸,酸) 的促进子区域.
在M. oryzae感染后,对选定的OsPITP基因的表达分析.

主要成果:

鉴定了30个OsPITP基因,表现出不同的特征.
OsPITP基因促进剂含有甲基斯酸 (Me JA) 和酸 (SA) 反应元素.
在M. oryzae感染时,特定的OsPITP基因 (OsML-1,OsSEC14-3,OsSEC14-4,OsSEC14-15,OsSEC14-19) 的表达显著改变.

结论:

OsPITPs代表了中的多样化家族,具有各种各样的特性.
有证据表明,OSPITPs可能在米天生的免疫力中发挥作用.
甲基斯酸和酸途径可能参与OsPITP介导的防御M. oryzae.

关键词:

我 JA 和 SA 是我的 JA 和 SA 这是一种Oryza sativa.这就是PITPs.具有天生的免疫力.

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