化,GA3和石墨烯氧化物如何影响菌体内建立和发展
Patrícia Duarte de Oliveira Paiva1, Diogo Pedrosa Correa da Silva1, Bruna Raphaella da Silva2
1Departamento de Agricultura, Escola de Ciências Agrárias, Universidade Federal de Lavras, Lavras 37200-000, MG, Brazil.
Plants (Basel, Switzerland)
|June 10, 2023
概括
斯特雷利兹植物的种子休眠状态可以通过用硫酸进行化学化来克服,在体外显著改善发芽率和苗木生长. 氧化石墨烯还增强了根茎和芽发育等特定的生长方面.
科学领域:
- 植物科学 植物科学
- 园艺园艺 园艺园艺
- 生物技术是生物技术.
背景情况:
- 通过体外培养进行斯特雷利齐亚植物的繁殖面临着挑战,原因是种子发芽时间延长和百分比低,主要是由于种子休眠而引起的.
- 有效的种子发芽策略对于优化体外繁殖至关重要,这是生产这些植物在受控条件下的可行的替代方案.
研究的目的:
- 评估化学 (硫酸) 和物理化方法与吉伯酸 (GA3) 结合在斯特雷利兹菌种子发芽和幼苗发育上的疗效.
- 研究石墨烯氧化物对斯特雷利兹植物体外培养的影响,评估其对生长和生化参数的影响.
主要方法:
- 种子经历了不同时间的硫酸化 (10-60分钟) 和物理化 (砂纸).
- 经过化和消毒后,种子在Murashige和Skoog (MS) 中培养,并补充了不同度的GA3.
- 另外一个实验涉及试管培养种子,其度不同,氧化石墨烯 (50和100毫克L-1).
- 测量包括发芽百分比,芽和根的长度,幼苗的存活率和抗氧化酶活动.
主要成果:
- 不管GA3的应用如何,在硫酸化30到40分钟的过程中,都能达到最高的发芽率.
- 在体外培养60天后,物理化和硫酸处理持续时间对芽和根的长度产生了积极的影响.
- 在没有GA3.3的硫酸浸泡30分钟和40分钟后,观察到最佳的苗木存活率.
- 50毫克L-1的石墨烯氧化物促进了根茎生长,而100毫克L-1增强了芽生长.
- 虽然马隆迪化物 (MDA) 水平没有受到影响,但抗氧化酶活性在对石墨烯氧化物度的反应中表现出波动.
结论:
- 用硫酸进行30-40分钟的化学化在克服斯特雷利兹种子休眠状态和促进体外发芽方面非常有效.
- 化技术和石墨烯氧化物都提供了有前途的策略,可以提高斯特雷利兹植物的繁殖效率和生长特征.
- 对石墨烯氧化物作用的进一步研究可能会导致植物组织培养中的新应用,以增强开发.
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