RsPORB的表达与卜根颜色有关
Da-Hye Kim1,2, Sun-Hyung Lim1,2, Jong-Yeol Lee3
1Division of Horticultural Biotechnology, School of Biotechnology, Hankyong National University, Anseong 17579, Republic of Korea.
Plants (Basel, Switzerland)
|June 10, 2023
概括
NADPH:protochlorophyllide氧化降解酶 (PORs) 是根中菌生物合成的关键. RsPORB基因促进体的变异解释了根色的差异,影响了植物的健康和外观.
科学领域:
- 植物生物学 植物生物学
- 生物化学 生物化学
- 遗传学 遗传学 是一个
背景情况:
- 叶子中的基生物合成机制已经得到了很好的研究,但在其他植物组织中了解得很少.
- 卜根的颜色变化与叶绿素和氨酸的积累有关,影响营养价值和视觉吸引力.
研究的目的:
- 调查NADPH:protochlorophyllide氧化还原酶 (PORs) 在根中的生物合成中的作用.
- 确定导致卜根绿色变色的遗传因素.
主要方法:
- 对绿色和白色卜根中的RSPORB基因表达水平的分析.
- 病毒诱导的基因沉默来评估RSPORB功能.
- 测序和对比 RsPORB 编码区域和不同卜线之间的促进者.
- 激发剂激活测试使用卜根原生质.
主要成果:
- 在绿色卜根中,rsPORB转录水平较高,与叶绿素含量呈正相关.
- RsPORB的编码区域在白色和绿色卜线上是相同的.
- 沉默RSPORB显著降低了叶绿素含量,证实了它在生物合成中的作用.
- 在RSPORB促进区域中,在白卜和绿卜之间发现了序列变化 (InDels和SNP).
- 促销商测试表明,RSPORB促销商中的InDels会影响其表达水平.
结论:
- RsPORB 是一种关键的酶,在非叶状组织 (如卜根) 中进行叶绿素生物合成.
- 在RSPORB促进体中的遗传变异是导致叶绿素积累和卜根色差异的原因.
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