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阿波利波蛋白A4在雄性小鼠中提高了交感活动和发热

Hsuan-Chih N Kuo^1,2, Zachary LaRussa^1,2, Flora Mengyang Xu³

¹Department of Biomedical Sciences, Heritage College of Osteopathic Medicine and Diabetes Institute, Ohio University, Athens, OH 45701, USA.

|June 10, 2023

概括

持续输注的阿波利波蛋白A4 (APOA4) 蛋白质可以促进棕色脂肪组织 (BAT) 热生成和肝脏脂肪酸氧化. 这可以改善甘油三水平和胰岛素敏感性,而不会影响体重或脂肪量.

科学领域:

代谢研究的研究.
内分泌学在内分泌学.
脂质代谢脂质的代谢

背景情况:

长链脂肪酸刺激阿波利波蛋白A4 (APOA4) 的产生和棕色脂肪组织 (BAT) 的热生成.
增加BAT热生成有助于甘油三清除,改善胰岛素敏感性.
之前的研究表明,急性APOA4给药可提高小鼠的BAT热生成.

研究的目的:

为了研究连续重组APOA4蛋白输液的生理作用.
评估APOA4对低脂肪饮食养小鼠的同情活动,发热和新陈代谢的影响.
测试APOA4是否在BAT和IWAT中增强同情活动和热生成,减少脂质,改善葡萄糖耐受性.

主要方法:

在小鼠中持续输注小鼠APOA4蛋白或盐水.
对同情活动的测量,BAT温度,能源消耗,体重和脂肪质量.
对葡萄糖耐受性,脂肪组织蛋白质,血脂质和肝脂肪酸氧化标记物的分析.

主要成果:

输注APOA4提高了BAT温度和热生成,并降低了血甘油三水平.
在BAT和肝脏中刺激了交感活动,但不是IWAT.
接受APOA4治疗的小鼠表现出肝脏脂肪酸氧化增加和肝脏甘油三酸含量降低.
观察到葡萄糖耐受性得到改善,葡萄糖挑战后血胰岛素水平降低.

结论:

不断输注APOA4蛋白质刺激了BAT和肝脏中的同情活性.
升高的BAT热生成和肝脂肪酸氧化导致血和肝脏甘油三水平降低.
APOA4治疗可以改善胰岛素敏感性,而不会改变热量摄入量,体重或脂肪质量.

关键词:

棕色脂肪组织棕色脂肪组织热量摄入量热量摄入量耐葡萄糖耐受性耐葡萄糖耐受性肝脏肝脏肝脏肝脏肝脏肝脏血脂质血脂质血脂质热生成是一种热生成.

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