老鼠中的酸盐和酸盐代谢:一项比较研究
Barbora Piknova1, Ji Won Park1, Samantha M Thomas1
1Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health, Bethesda, MD 20892, USA.
Nutrients
|June 10, 2023
概括
老龄化减少了氧化 (NO) 的可用性. 虽然老老鼠的酸盐水平增加,酸盐水平降低,但酸盐降低途径仍然是功能性的,这表明NO合成或信号发生了变化.
科学领域:
- 生理学 生理学 生理学
- 生物化学 生物化学
- 衰老研究研究 衰老研究
背景情况:
- 氧化 (NO) 对于生理调节至关重要,由于其不稳定性,可按需合成.
- 当地氧气水平决定了通过氧化合成酶 (NOS) 或酸盐/酸盐还原来产生的NO.
- 骨肌肉的酸盐储存支持局部和全身NO的可用性,随着年龄的增长而下降.
研究的目的:
- 调查各种老鼠组织中酸盐和酸盐水平与年龄相关的变化.
- 检查老化对酸盐运输和还原途径的影响.
- 为了确定与年龄相关的氧化可用性下降的潜在来源.
主要方法:
- 对年轻和老老鼠组织中酸盐和酸盐含量的比较分析.
- 评估不同年龄段的酸盐转运蛋白和酸盐还原酶水平.
- 在两个年龄组中,在饮食中补充酸盐后,对器官中酸盐丰富的评估.
主要成果:
- 与年轻大鼠相比,老老鼠的基线酸盐水平较高,酸盐水平较低.
- 大多数酸盐运输蛋白和酸盐还原酶水平在不同年龄组之间是相似的,除了眼睛.
- 饮食中酸盐补充剂导致老老鼠器官中酸盐的积累增加,这表明酸盐减少途径不受影响.
结论:
- 衰老会改变内源性酸盐和酸盐水平,但酸盐降解途径似乎是功能性的.
- 与年龄相关的氧化 (NO) 可用性下降可能源于氧化合成酶 (NOS) 活性变化或下游信号通路 (sGC/PDE5) 的变化.
- 需要进一步的研究,以阐明与年龄相关的NO可访问性变化背后的确切机制.
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