微流体生物传感器基于二硫化物 (MoS) 修改的薄芯微纤维用于免疫检测Toxoplasma gondii
Huiji Chen1, Binbin Luo1, Shengxi Wu2
1Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing 400054, China.
Sensors (Basel, Switzerland)
|June 10, 2023
概括
这项研究引入了一种新的微流体生物传感器,用于检测Toxoplasma gondii (T. gondii). 生物传感器使用涂有二硫的薄芯微纤维,为T. gondii检测提供高灵敏度和特异性.
科学领域:
- 生物医学工程 生物医学工程
- 寄生虫学的寄生虫学
- 纳米材料科学 科学 纳米材料科学
背景情况:
- 毒淋巴菌 (T. gondii) 对公众和动物健康构成重大威胁.
- 准确有效的T. gondii检测方法对于疾病控制和管理至关重要.
研究的目的:
- 开发和描述一种新的微流体生物传感器,用于敏感和特定的T. gondii. 免疫检测.
- 在检测范围,检测极限和特异性方面评估生物传感器的性能.
主要方法:
- 通过将单模纤维与TCMF融合,制造一个薄芯微纤维 (TCMF) 生物传感器.
- 将TCMF封装在微流体芯片中,以保护和避免干扰.
- 用二硫化物 (MoS2) 和T. gondii抗原对TCMF表面进行修改,用于免疫检测.
主要成果:
- 生物传感器显示T. gondii单克隆抗体溶液的检测范围为1 pg/mL至10 ng/mL.
- 获得了高灵敏度3.358nm/log (mg/mL) 和低检测极限87fg/mL.
- 使用狂犬病病毒,伪狂犬病病毒和T. gondii血清证实了出色的特异性.
结论:
- 拟议的MoS2涂层TCMF微流体生物传感器是T. gondii检测的高度敏感和特定的工具.
- 该生物传感器在生物医学领域,特别是诊断领域的应用具有显著的潜力.
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