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尿路生物标志物的个体内变化,住院儿童与球体疾病:一个前性观察性研究
Jianmei Zhou1, Xuhui Zhong1, Huijie Xiao1
1Department of Pediatrics, Peking University First Hospital, Beijing, China.
European journal of pediatrics
|June 10, 2023
概括
尿路生物标志物显示出患有球体疾病的住院儿童的日间变化. 表皮生长因子 (EGF) 是一个稳定的生物标志物,不受采集时间或处理的影响.
科学领域:
- 儿科脏病学 儿科脏病学
- 临床化学 临床化学
- 生物标志物研究 生物标志物研究
背景情况:
- 尿路生物标志物对于诊断和管理儿科球细胞疾病至关重要.
- 了解这些生物标志物的个体内变异对于准确的临床解释至关重要.
- 以前的研究还没有完全阐明收集时间和样本处理对住院儿童尿路生物标志物的影响.
研究的目的:
- 评估被诊断患有淋巴细胞疾病的住院儿童尿路生物标志物的个体内变化.
- 评估日间节律和样本处理对常见和新型尿路生物标志物的影响.
- 为了确定表皮生长因子 (EGF) 在这个人群中作为尿路生物标志物的稳定性和可靠性.
主要方法:
- 从患有质细胞疾病的住院儿童收集过夜和24小时的尿液样本.
- 测量蛋白质,白蛋白,N-乙-β-D-葡萄糖胺酶和EGF的度,通过肌素,度或比重进行正常化.
- 评估了白天变化以及样品离心,添加剂,储存温度和延迟处理对生物标志物稳定性的影响.
主要成果:
- 肌素正常化生物标志物在不同的24小时采集周期中显示出更好的一致性.
- 对尿蛋白,白蛋白,N-乙-β-D-葡萄糖胺酶和EGF观察到显著的日间变化.
- 尿液EGF表现出较低的日内和日间变异性,不受样本处理方法的影响,与24小时度有很好的一致性.
结论:
- 尿中的生物标志物度在患有淋巴细胞疾病的住院儿童中表现出白天变化.
- 在临床实践中建议采集标准化尿液时间,以尽量减少变化.
- 尿液EGF成为未来儿科脏病学临床应用的稳定可靠生物标志物.
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