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使用人类诱导的神经细胞测量神经网络活动.

Danny McSweeney1,2, Jay English1,2, Ethan Howell2

  • 1Graduate Program in Molecular and Cellular Biology, University of Massachusetts Amherst, Amherst, MA, USA.

Methods in molecular biology (Clifton, N.J.)
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概括

本研究详细介绍了在高密度微电极阵列 (HD-MEAs) 上培养人类诱导神经元 (iNs) 的方法,以观察成熟网络活动. 它为研究人员提供了关于神经元网络信号和故障排除的见解.

关键词:
活动测试试验.互补金属氧化物半导体 (CMOS) 芯片技术技术高密度微电极阵列 高密度的微电极阵列.神经网络测定 神经网络测定在 Ngn2-iNs 中.

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科学领域:

  • 神经科学是一个神经科学.
  • 干细胞生物学 干细胞生物学
  • 生物技术是生物技术.

背景情况:

  • 同步的神经元发射,或网络爆发,表明成熟的神经元网络.
  • 之前的工作在2D人类神经元模型中确定了这种现象.
  • 人类多能干细胞 (hPSCs) 为研究神经元发育和功能提供了一个模型.

研究的目的:

  • 描述在高密度微电极阵列 (HD-MEAs) 上培养人类诱导神经元 (iNs) 的方法.
  • 在体外分析神经元网络活动模式.
  • 为使用HD-MEAs的研究人员提供故障排除指导.

主要方法:

  • 皮层刺激性INs与hPSCs有所区分.
  • 在HD-MEAs上涂抹了NN,并培养到成熟.
  • 使用HD-MEA技术记录和分析神经元活动.

主要成果:

  • 介绍了来自人类野生类型 Ngn2-iN 的代表性数据.
  • 这项研究证明了在NN种文化中观察HD-MEAs网络活动的可行性.
  • 在之前的突变状态研究中观察到网络信号中的不规则.

结论:

  • 描述的方法使得使用INs和HD-MEAs研究成熟的人类神经网络.
  • 这种方法有助于研究神经元网络动态和潜在的信号缺陷.
  • 这些发现支持使用iN-HD-MEA系统进行神经学研究.