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E2F2通过呈现"E2F2/miR-16-5p/SPTLC1"方案,作为人类细胞癌的基本预后生物标志物和治疗标

GenYi Qu¹, Guang Yang¹, Dan Chen¹

¹Department of Urology, ZhuZhou central hospital, ZhuZhou, Hunan Province 412000, China.

Translational oncology

|June 10, 2023

概括

转录因子E2F2通过激活miR-16-5p促进细胞癌 (RCC) 的进展,然后抑制SPTLC1.1. 这个E2F2/miR-16-5p/SPTLC1轴是RCC的潜在生物标志物.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

细胞癌 (RCC) 是一种流行且致命的尿道系统恶性瘤.
细胞周期转录因子E2F2在RCC瘤发生中的作用尚不清楚.
在RCC开发中,E2F2的精确下游信号通路需要阐明.

研究的目的:

研究E2F2在细胞癌 (RCC) 进展中的作用.
为了识别下游的信号轴,在RCC中通过E2F2调节.
评估RCC中的E2F2/miR-16-5p/SPTLC1轴的预后和治疗潜力.

主要方法:

对E2F2,SPTLC1和miR-16-5p表达模式和预后意义的TCGA数据库的分析.
在38个配对的RCC组织中使用RT-qPCR和Western blot进行验证.
在体外功能测试 (MTT,EDU,殖民地形成,Transwell) 和体内异种移植模型.
染色体免疫沉和光酶记者测定以确定调节电路.

主要成果:

在RCC组织和细胞中,E2F2表达显著升高,与较短的整体存活率相关.
E2F2通过转录激活miR-16-5p,导致SPTLC1表达的负调节.
击败E2F2抑制了RCC细胞生物功能,这些生物功能被miR-16-5p模仿物所拯救,并进一步被SPTLC1过度表达所废除.
证实了E2F2/miR-16-5p/SPTLC1轴在体外和体内驱动RCC瘤发生.

结论:

E2F2通过miR-16-5p/SPTLC1信号轴促进RCC的进展.
这个轴代表了细胞癌的新预后生物标志物.
E2F2/miR-16-5p/SPTLC1通路可能成为RCC的潜在治疗点.

关键词:

E2F2 E2F2 E2F2 E2F2 E2F2 E2F2 细胞癌是细胞癌.在SPTLC1中,在 miR-16 5pp 中.

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