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新型定制的年龄依赖的角膜膜和与装载着德布洛芬的可生物降解纳米颗粒的相互作用

Gerard Esteruelas¹, Alba Ortiz¹, Josefina Prat¹

¹Department of Pharmacy, Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, 08028 Barcelona, Spain; Institute of Nanoscience and Nanotechnology (IN2UB), University of Barcelona, 08028 Barcelona, Spain.

Colloids and surfaces. B, Biointerfaces

|June 10, 2023

概括

装有德克西布洛芬 (DXI-NPs) 的聚乳-同-甘油酸 (PLGA) 纳米颗粒显示改善了眼部药物输送. 这些DXI-NP与角膜脂质膜相互作用,并表现出与年龄相关的相互作用差异,表明有针对性的眼疗法的潜力.

科学领域:

眼科医生眼科眼科
材料科学材料科学材料科学
生物物理学的生物物理.

背景情况:

眼部炎症是常见的,用低生物可用性的德西布洛芬 (DXI) 眼滴治疗.
聚乳糖糖酸 (PLGA) 纳米颗粒为眼部应用提供了增强的药物输送.
与年龄相关的角膜变化通常不会被当前的抗炎眼滴配方解决.

研究的目的:

为了研究与角膜模型装载德西布洛芬PLGA纳米粒子 (DXI-NP) 的相互作用机制.
评估DXI-NP与角膜脂质膜相互作用的年龄相关差异.
评估DXI-NP用于眼部药物输送的体外和体内行为.

主要方法:

开发可代表成人和老年人群的角膜模型 (脂质单层,囊泡).
利用兰木尔平衡,二极电位,异极性和共聚焦显微镜来研究DXI和DXI-NP相互作用.
在小鼠体内使用光标记纳米颗粒的体内研究.

主要成果:

通过粘附和包裹过程,DXI-NP粘附于脂质膜的刚性区域.
在老年角膜模型中增加的膜刚性会影响DXI-NP相互作用和二极电位.
在体外和体外数据证实了DXI-NP对有序脂质相的坚持,并观察到与年龄相关的差异.

结论:

DXI-NPs显示与角膜脂质膜的有效相互作用,显示了改善眼部药物递送的潜力.
角膜膜的年龄相关变化会影响DXI-NP相互作用.
该研究提供了关于DXI-NP与角膜组织相互作用的机制的见解,考虑到与年龄相关的变化.

关键词:

可生物降解的纳米粒子.角膜膜模型的角膜模型德克西布洛芬 (dexibuprofen) 是一种药物.兰木尔的单层材料是Langmuir的一层.脂质体是一种脂质体.

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