在中国三种中西藏人群中对74种微型和法医特征进行了大规模并行测序
Jing Liu1, Yonglin Su2, Yufeng Wen3
1Institute of Forensic Medicine, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China.
Forensic science international. Genetics
|June 10, 2023
概括
这项研究验证了在中国人群中用于法医遗传学的新型微型型组. 该面板显示了祖先推断和混合物解卷的优秀性能.
科学领域:
- 法医遗传学 法医遗传学
- 人口遗传学 人口遗传学
- 人类识别 人类识别
背景情况:
- 微型 (MHs) 是新兴的法医遗传标记,具有混合解卷和祖先推断的潜力.
- 评估新型的MH面板对于推进法医应用至关重要.
研究的目的:
- 评估Ion AmpliSeq MH-74 Plex微哈普洛型研究小组在中国三种中西藏人群中的性能和实用性.
- 通过使用 MH 数据,调查这些群体内的人口关系和祖先组成部分.
主要方法:
- 使用Ion AmpliSeq MH-74 Plex面板和Ion Torrent测序,对74个微型的基因型定型.
- 对测序性能,等位基因频率,有效等位基因数 (Ae) 和信息性 (In) 的分析.
- 主要成分分析 (PCA) 和结构分析在人口遗传学中的应用.
主要成果:
- MH面板展示了强大而可靠的测序性能.
- 观察到高多态性,75.68%的MH具有有效的等位基因数 (Ae) >2.0000.00.
- 在汉族,藏族和易族人群中发现了显著的等位基因频率变异,藏族和易族人群之间更为密切的遗传亲和关系.
结论:
- 离子AmpliSeq MH-74 Plex 微哈普洛型研究小组是研究中国人群中人类法医学的高度多态和有效工具.
- 该小组显示了大陆人口分层的潜力,但需要进一步发展,以获得更精细的亚种群解决方案和全面的数据库.
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