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LncRNA-AK007111通过调节乳腺细胞功能来影响喘中的呼吸道炎症

Jiejing Xu¹, Siqing Zhang², Huilin Li³

¹Children's Hospital of Nanjing Medical University, Nanjing, Jiangsu, China; Department of Pediatrics, The Second People's Hospital of Changzhou, Affiliate Hospital of Nanjing Medical University, Changzhou, Jiangsu, China.

International immunopharmacology

|June 10, 2023

概括

长非编码RNA-AK007111 (lncRNA-AK007111) 通过促进炎症和呼吸道过度反应,使喘恶化. 它的下调抑制了巨细胞脱粒和迁移,为喘提供了潜在的治疗点.

科学领域:

分子生物学分子生物学
免疫学免疫学免疫学
呼吸系统医学呼吸系统医学

背景情况:

长非编码RNAs (lncRNAs) 参与基因调节和疾病发病.
众所周知,几种lncRNAs可以影响喘的发展.
在喘中lncRNA-AK007111的特定作用仍然在很大程度上未被探索.

研究的目的:

在喘的背景下研究新型lncRNA-AK007111的功能.
阐明lncRNA-AK007111通过哪些分子机制影响喘病理,特别是巨细胞活动.

主要方法:

喘小鼠模型由卵蛋白敏感化诱导.
对lncRNA-AK007111过度表达和淘汰的病毒传染.
对支气管支气管洗液和肺组织进行炎症标志物的分析.
使用动物肺功能分析仪评估气道过敏反应.
免疫光用于大细胞检测和脱粒测定 (β-hexosaminidase释放).
对于量化IL-6和TNF-α的ELISA.
使用RBL-2H3巨细胞进行体外研究.

主要成果:

在喘模型中,lncRNA-AK007111的升调促进了炎症细胞的透,增加了巨细胞数量,提高了IL-5和IL-6水平,并恶化了气道过度反应.
低调 lncRNA-AK007111 降低了巨细胞脱粒化,抑制了IL-6和TNF-α的表达,并影响了巨细胞的迁移.
lncRNA-AK007111直接影响杆细胞脱粒和迁移.

结论:

lncRNA-AK007111在喘病原发生过程中起着重要作用.
lncRNA调节乳腺细胞功能,包括脱粒和迁移,有助于呼吸道炎症和过度反应.
lncRNA-AK007111代表了治疗喘的潜在治疗标.

关键词:

气道气道气道炎症炎症是一种炎症.乳腺细胞的功能 OVA诱导的喘小鼠模型在cRNA-AK007111中.

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