类似转录激活器的效应器保护细菌内生生物从菌菌中陷入困境
Ingrid Richter1, Philipp Wein1, Zerrin Uzum1
1Department of Biomolecular Chemistry, Leibniz Institute for Natural Product Research and Infection Biology, HKI, Beutenbergstr. 11a, 07745 Jena, Germany.
Current biology : CB
|June 10, 2023
概括
一种类似细菌转录激活器 (TAL) 的效应器使Mycetohabitans rhizoxinica能够逃避真菌防御,并在Rhizopus microsporus中迁移. 没有TAL效应器,细菌就会被困,它们的生存率就会降低,这揭示出一种新的细菌共生策略.
科学领域:
- 微生物学 微生物学
- 菌类学 菌类学是指菌类学.
- 细菌遗传学 细菌遗传学
背景情况:
- 菌群住户Rhizoxinica是真菌Rhizopus microsporus的一个内生生物.
- 在真菌菌中,M. rhizoxinica的导航和生存机制在很大程度上是未知的.
- 促进细菌-真菌共生的细菌效应对宿主-微生物相互作用至关重要.
研究的目的:
- 确定使M. rhizoxinica能够在R. microsporus中迁移的细菌因素.
- 调查细菌效应剂在内共生体生存和宿主防御逃避中的作用.
- 阐明了M. rhizoxinica-R. microsporus共生关系背后的分子机制.
主要方法:
- 微流体和光显微镜观察细菌行为.
- 高分辨率的真菌和内分泌细菌的实时成像.
- 活/死染色,以评估细菌的生存能力.
主要成果:
- 一种类似转录激活器 (TAL) 的效应因子对于M. rhizoxinica的共生至关重要.
- 缺乏TAL的细菌的迁移减少,并富含侧.
- 被困在TAL缺乏的细菌的细胞内生存率显著降低.
- 缺少TAL蛋白引发了对细菌的保护性宿主反应.
结论:
- 塔尔效应因子对于内共生生物的生存和在真菌菌中殖民至关重要.
- 菌根菌使用TAL效应器来破坏宿主防御机制.
- 这项研究揭示了TAL效应因子在细菌内共生和宿主微生物相互作用中的前所未有的功能.
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