具有16S-PICRUSt2推断或直接acdS基因测序的土壤中的含有细菌的ACC除氨酶的社区结构和丰度

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Updated: Oct 27, 2026

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

Daniel K Manter¹, Alison K Hamm¹, Heather L Deel¹

¹United States Department of Agriculture, Agricultural Research Service, Soil Management and Sugarbeet Research Unit, 2150 Centre Ave Bldg D, Fort Collins, CO 80526, USA.

Journal of microbiological methods

|June 10, 2023

概括

识别促进植物生长的细菌的非培养方法是关键. 这项研究比较了qPCR和16SrRNA测序,发现两者都随着土壤湿度下降而下降,但与不同的细菌群体识别有所不同.

科学领域:

微生物生态学微生物生态学
植物与微生物的相互作用
分子生物学分子生物学

背景情况:

具有1-氨基cyclopropane-1-carboxylate deaminase (ACCD +) 的细菌通过调节乙烯水平来增强植物的应激弹性.
对ACCD+细菌的有效清单受到开发不足的非培养基方法的限制.
土壤水的可用性影响微生物社区的结构和功能.

研究的目的:

为了比较两种独立于培养的方法来识别和量化土壤中的ACCD+细菌.
评估在不同的水资源供应条件下,ACCD+细菌的数量和社区结构.
评估使用PICRUSt2对qPCR和16SrRNA测序的实用性,用于微生物组的功能概况.

主要方法:

使用新型acdS基因特异性原料的定量PCR (qPCR).
使用PICRUSt2.2进行16S rRNA amplicon测序和遗传学分析.
在科罗拉多州东部的水供应梯度上采集土壤样本.

主要成果:

qPCR和PICRUSt2在ACCD+基因丰度中显示出显著的相关性.
PICRUSt2在Acidobacteriota,Pseudomonadota和Bacteroidota族群中发现了ACCD+细菌;acdS原料只检测到了Pseudomonadota.
随着土壤水含量减少,ACCD+细菌的丰富性随着潜在的蒸发转化梯度的降低而减少.

结论:

使用PICRUSt2进行qPCR和16SrRNA测序对研究ACCD+细菌有价值.
16S rRNA测序和PICRUSt2提供了土壤微生物群的更广泛的功能概况.
类型识别中的差异凸显了功能基因研究中需要谨慎选择方法的需要.

关键词:

1-氨基cyclopropane-1-carboxylate 脱氨酶的使用情况.16S rRNA amplicon 序列的测序方式遗传学上的推断.定量PCR是一种定量PCR.

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