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转体子有助于在大脑中获得细胞类型特定的cis元素

Kotaro Sekine^1,2, Masahiro Onoguchi^3,4, Michiaki Hamada^5,6,7

¹Graduate School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.

Communications biology

|June 10, 2023

概括

可转移元素 (TE) 已经演变为对大脑功能至关重要的调节性DNA元素. 特定的TE衍生的元素,MER130和MamRep434,在神经元基因调节和大脑进化中起作用.

科学领域:

神经科学是一个神经科学.
进化生物学进化生物学
基因组学就是基因组学.

背景情况:

哺乳动物大脑的进化涉及复杂的基因调节.
可转移元素 (TE) 越来越多地被认为是功能性 cis-regulatory 元素.
对于TEs在脑特异性基因调节网络中的确切作用仍然不完全理解.

研究的目的:

通过单细胞分析,识别TE衍生的对特定脑细胞类型至关重要的cis-regulatory元素.
调查这些TE衍生元素的进化历史和功能意义.

主要方法:

用于转移酶可访问染色体测序的单细胞测试 (scATAC-seq) 用于公共数据集.
分析的重点是发现TE衍生的cis元素及其相关的转录因子基因.
使用比较基因组学来追踪已识别的TE家族的进化放大.

主要成果:

来自MER130和MamRep434可转移元素的DNA元素作为转录因子结合点起作用.
这些元素中包含了Neurod2和Lhx2的基因,特别是在谷氨酸性神经元前体中.
分别MER130和MamRep434的cis元素在Amniota和Eutheria的祖先中显示了放大.

结论:

在不同的进化阶段,TEs贡献了 cis 调节元素,影响了大脑的复杂性.
来自TE的cis元素的进化可能在获得不同的大脑功能和形态学方面发挥了作用.
这项研究强调了可移植元素对哺乳动物大脑进化和基因调节的重大影响.

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