附着的filopodia的基隆生长
Wenhong Li1, Wen-Lu Chung2, Michael M Kozlov3
1Department of Immunology, Weizmann Institute of Science, Rehovot, Israel.
Biophysical journal
|June 11, 2023
概括
细胞体表现出形曲,由于行为动力学,以左形状延伸. 这种定向的细胞突起受到特定的与行为相关的蛋白质的影响,例如myosin-X和formin DAAM1.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 细胞骨动力学 细胞骨动力学
背景情况:
- 菲洛波迪亚是动态的细胞膜突起,对细胞粘附,迁移和感知至关重要.
- 它们的形成和延长是由actin细胞骨的聚合驱动的.
- 了解filopodia的定向延伸是解读细胞导航的关键.
研究的目的:
- 为了研究filopodia的方向延伸背后的机制.
- 为了识别涉及到filopodia的性曲的分子参与者.
- 探索与行为因相关的蛋白质在产生细胞不对称性中的作用.
主要方法:
- 在盖莱-8涂层基板上的细胞培养.
- 低温电子断层扫描用于高分辨率成像的filopodia结构.
- 动氨酸相关蛋白质表达的遗传调节.
- 药理上抑制细胞粘附.
主要成果:
- 在galectin-8基板上形成的附着filopodia显示出一个一致的左侧曲的形形状.
- 低温电子断层扫描显示,在向左转动时,actin核心束位移到filopodia中线的右边.
- 抑制加勒-8的附着性取消了菲洛波迪亚的性.
- 确定了myosin-X和formin DAAM1作为filopodia性的主要促进者,formin mDia1,VASP和fascin也发挥了作用.
结论:
- 乙细胞骨架和有限的一组相关蛋白质可以产生复杂的细胞导航行为.
- 菲洛波迪亚的左右不对称性是指向细胞突起的表现.
- 菲洛波迪亚的性代表了细胞环境感知和响应的基本机制.
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