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循环免疫细胞的蛋白质组分析确定了与COVID-19严重程度相关的细胞表型

Martin Potts¹, Alice Fletcher-Etherington¹, Katie Nightingale¹

¹Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, UK; Department of Medicine, University of Cambridge, Cambridge CB2 0QQ, UK.

|June 11, 2023

概括

新的研究确定了与严重的COVID-19相关的特定细胞表面蛋白质. 周围血液单核细胞 (PBMC) 上的这些标记物可以帮助患者评估和治疗向.

科学领域:

免疫学免疫学免疫学
蛋白质组学是指蛋白质组学.
病毒学病毒学

背景情况:

像C-反应蛋白 (CRP) 和D-二次体这样的血清蛋白在严重急性呼吸综合征冠状病毒2 (SARS-CoV-2) 中具有预后价值,但缺乏特异性.
这些非特异性标志物对驱动严重COVID-19病原体的外周血液单核细胞 (PBMC) 种群提供了有限的洞察力.

研究的目的:

通过公正的蛋白质组分析,识别与COVID-19严重程度相关的细胞表型.
建立与疾病进展相关的综合性多基因特征.

主要方法:

来自40名未接种疫苗的SARS-CoV-2患者的总和血膜PBMC蛋白质的综合分析.
整合蛋白质组数据与RNA测序 (RNA-seq) 和来自同一捐赠者的流细胞计.

主要成果:

免疫细胞失调随着COVID-19疾病严重程度的增加而进展.
细胞表面蛋白CEACAMs (1, 6, 8),CD177,CD63和CD89与严重的COVID-19密切相关.
在严重病例中出现不典型的单核细胞群 (CD3+CD4+CEACAM1/6/8+CD177+CD63+CD89+和CD16+CEACAM1/6/8+).

结论:

特定的细胞表面蛋白质标记物可以在严重的COVID-19中表征免疫细胞表型.
这些标记物可以通过流细胞计进行实时患者评估.
鉴定这些免疫群体可能会导致针对COVID-19免疫病理学的向治疗.

关键词:

CP: 免疫学免疫学

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