来自一种致病性菌Inonotus obliquus菌株IO-B2的素降解酶
Retno Agnestisia1,2,3, Tomohiro Suzuki4,5, Akiko Ono6
1United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan.
AMB Express
|June 11, 2023
概括
一种传统医药,Inonotus obliquus,通过基因组测序来研究其寄生虫生命周期. 研究人员确定了过氧化酶 (MnP) 基因,并特征了一种新的MnP,IoMnP1,对木材降解至关重要.
科学领域:
- 菌类学 菌类学是指菌类学.
- 生物化学 生物化学
- 基因组学就是基因组学.
背景情况:
- 形菌 (Inonotus obliquus) 是一种具有病原性真菌,具有传统医疗用途,特别是用于癌症治疗.
- 它的寄生虫生命周期和在宿主感染中的作用,涉及菌素降解酶,仍然不完全理解.
研究的目的:
- 为了研究 I. obliquus 中的 laccase (Lac),过氧化酶 (MnP) 和红素过氧化酶 (LiP) 的活性.
- 通过基因组测序识别与木材退化相关的基因.
- 为了描述来自I. obliquus. 的假定过氧化 (MnP) 的特征.
主要方法:
- 在柯克的媒介中培养I. obliquus.
- 基因组测序和I. obliquus. 的分析.
- 克隆和分子表征一个假定的MnP (IoMnP1).
- 对IoMnP1.1.的遗传学分析
主要成果:
- I. obliquus的基因组包含21,203个预测的蛋白质编码基因,其中134个与木材降解有关.
- 有相当多的基因 (47) 与红素降解有关,包括多个mnp基因.
- 一种新的MnP,IoMnP1,被确定和描述,表现出与MnP活动一致的催化特性.
- 遗传学分析将IoMnP1置于Hymenochaetaceae家族中,与其他已知的MnPs密切相关.
结论:
- 在Inonotus obliquus中,IoMnP1被确定为一个功能性过氧化酶 (MnP).
- 这项研究提供了关于I. obliquus.木材退化的遗传基础的见解.
- 了解这些酶可能会阐明I. obliquus的寄生机制.
更多相关视频
08:31Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
Published on: October 3, 2018
8.5K
08:21Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials
Published on: May 16, 2022
4.9K
相关概念视频
Fungal Group Zygomycota
Zygomycota, previously classified as a distinct fungal group, are primarily terrestrial, saprophytic molds that play a crucial role as decomposers. Recent phylogenetic studies have revealed that these fungi are now divided into two major clades — Mucoromycota, which includes many symbiotic species, and Zoopagomycota, which primarily consists of parasitic and pathogenic fungi. These groups exhibit distinct ecological roles and reproductive strategies while sharing key structural and...
Biodeterioration
Biodeterioration refers to the unwanted alteration of materials caused by microorganisms—especially fungi—which damage both organic substrates (paper, wood, textiles) and inorganic ones (stone, plaster, glass). Unlike abiotic decay, biodeterioration results from biological activity that produces physical disruption and chemical degradation.Physical deterioration occurs as fungal hyphae penetrate pores, cracks, and surface irregularities. Hyphal turgor pressure, thigmotropic growth along...
Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
