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菌 F₁-ATPase 的冷-EM 结构

Chui-Fann Wong¹, Chen-Yen Leow¹, Gerhard Grüber¹

¹Nanyang Technological University, School of Biological Sciences, 60 Nanyang Drive, Singapore, 637551, Republic of Singapore.

Biochemical and biophysical research communications

|June 11, 2023

概括

非结核菌肺部疾病正在上升. 研究人员净化和结构性地描述了Mycobacterium abscessus F1-ATPase复合体,这是一个潜在的药物标,揭示了其低的内在活性.

科学领域:

生物化学生物化学
结构生物学结构生物学
微生物学微生物学

背景情况:

由Mycobacterium abscessus (Mab) 引起的肺部疾病病例的增加需要新的治疗点.
氧化酸化途径及其ATP合成酶是Mab抑制剂的有吸引力的标.

研究的目的:

为了产生和净化一个重组的,活跃的Mycobacterium abscessus F1-ATPase复合体 (MabF1-αβγδε).
为了获得对Mab F1-ATPase的机制,监管和结构洞察力.
确定第一个Mab F1-ATPase复合物的冷电子显微镜结构.

主要方法:

再组合的MabF1-αβγδε复合物的表达和净化.
电子显微镜用于结构的确定.
酶分析测量ATP水解活性.

主要成果:

获得了高度纯净和酶活性的MabF1-αβγδε复合物.
马布F1-ATPase的第一个冷电子显微镜结构在7.3 Å分辨率下确定.
该酶表现出较低的基底ATP水解活性,而素治疗增强了这种活性.

结论:

描述的Mab F1-ATPase复合体为了解其功能和开发抑制剂提供了基础.
对Mab F1-ATPase的结构洞察力可以指导新型抗菌菌药物的设计.
向F1-ATPase是治疗非结核性菌根性肺部感染的一个有希望的策略.

关键词:

这是ATP合成酶.生物能源学生物能源学电子显微镜的电子显微镜传染性疾病传染性疾病这是一种Mycobacterium细菌.非结核性菌根杆菌是没有结核的.

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