直接利用和转化原粉到外聚糖由一个新分离的氨溶性Streptococcus sp
Reeba Parameswaran1, M P Soumya2, K Madhavan Nampoothiri1
1Microbial Processes and Technology Division, CSIR, National Institute for Interdisciplinary Science and Technology (CSIR-NIIST), Trivandrum 695019, Kerala, India.
Journal of biotechnology
|June 11, 2023
概括
流球菌 (Streptococcus lutetiensis) 从粉中产生德克斯外聚糖化物 (EPS). 这种新的方法有效地将低成本的粉转化为稳定的EPS,无需外部酶,提高经济可行性.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 生物化学 生物化学
背景情况:
- 一种新型的微生物菌株Streptococcus lutetiensis被分离出来.
- 该菌株表现出显著的粉糖化和粉溶解活性.
- 它还证明了粉介质中的大量外聚糖 (EPS) 生产.
研究的目的:
- 确定和描述一种用于产出外聚糖 (EPS) 的新型微生物来源.
- 从低成本的粉材料优化EPS的生产.
- 分析产生的EPS的特性和结构.
主要方法:
- 隔离和识别了Streptococcus lutetiensis. 的一种细菌.
- 用麻粉优化EPS生产的发酵条件.
- 使用单糖类分析,FT-IR,TGA,GPC NMR 和 SEM 净化和表征EPS.
- 对粒子大小和泽塔潜力的分析.
主要成果:
- 从麻粉中获得了19.92 ± 0.5 g/L的最大EPS产量.
- 纯化的EPS被确定为德克斯,其分子量 (Mw) 为1275.36kDa.
- 该EPS表现出稳定性,在性条件下随机卷轴形状,以及剪切稀释特性.
结论:
- 杆菌 lutetiensis 是一个有前途的候选人,用于高效的德克斯外聚糖化物 (EPS) 生产.
- 粉材料的一步转化为EPS生物合成提供了一种经济可行的方法.
- 具有特征的EPS具有适用于各种生物技术应用的特性.
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