Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

1.5K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.5K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

830
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
830

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Genome-Wide Characterization of CAPE-Producing <i>PR1</i> Genes Reveals Regulator-Dependent Expression and Abiotic Stress-Associated Functions in <i>Nicotiana tabacum</i>.

Plants (Basel, Switzerland)·2026
Same author

Artificial Oxidation: A Major Challenge in Implementing Multi-Attribute Methods for Therapeutic Protein Analysis.

Pharmaceuticals (Basel, Switzerland)·2026
Same author

Targeted next-generation sequencing confirms rabies in a patient with unknown exposure: a case report.

Frontiers in medicine·2026
Same author

Flurbiprofen Axetil Combined with Ultrasound-Guided Intercostal Nerve Block for Preoperative Analgesia in Patients with Traumatic Multiple Rib Fractures: A Randomized Controlled Trial.

Pain and therapy·2026
Same author

Synergistic High Entropy and Inductive Effects in Aerogels Accelerate pH-Universal Hydrogen Evolution.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

GmERF1 positively regulates soybean resistance to Phytophthora sojae by enhancing salicylic acid signaling.

Plant cell reports·2025

相关实验视频

Updated: Jul 27, 2025

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

4.9K

检测两次LC-MS运行之间的罕见差异的方法.

Zhongqi Zhang1, Jason Richardson1, Bhavana Shah1

  • 1Process Development, Amgen Inc., One Amgen Center Drive, Thousand Oaks, CA, 91320, USA.

Analytical biochemistry
|June 11, 2023
PubMed
概括

一种新的统计方法检测了生物制药样本之间罕见的差异,没有复制品. 这种方法通过减少假阳性和假阴性来改善多属性方法 (MAM) 的新峰值检测.

科学领域:

  • 生物制药学分析分析
  • 分析化学是一种分析化学.
  • 质谱测量质量谱测量

背景情况:

  • 基于LC-MS的多属性方法 (MAM) 对于监测生物制药质量属性至关重要.
  • 检测新的或缺失的峰值对于MAM实施和比较分析至关重要.
  • 由于信号强度依赖于MS的变化性,比较没有复制品的类似样本具有挑战性.

研究的目的:

  • 开发一种统计方法,用于检测两个非常相似的样本之间的罕见差异.
  • 为了使样品可靠地比较,而不需要重复分析.
  • 提高生物制药质量控制中新峰值检测的准确性.

主要方法:

  • 开发了一种新的统计方法,假设大多数组件在样本之间是同等的.
  • 该方法考虑了信号强度相关的相对变化.
  • 分析了单克隆抗体片映射数据集,以验证该方法.

主要成果:

  • 该统计方法成功地检测出高度相似的样本之间的罕见差异.
  • 该方法证明适合在MAM中检测新峰值.
  • 在没有大幅增加虚假负面率的情况下,实现了虚假阳性率的显著降低.
关键词:
错误发现率 错误发现率一个错误的负面.这是错误的阳性.质谱测量质量谱测量多属性方法多属性方法.新峰值检测检测的新峰值检测

更多相关视频

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

22.6K
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

6.9K

相关实验视频

Last Updated: Jul 27, 2025

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

4.9K
Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

22.6K
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

6.9K

结论:

  • 开发的统计方法提供了一个强大的解决方案,用于识别复杂样本中的微妙变化.
  • 这种技术提高了MAM在生物制药特征表征方面的可靠性.
  • 该方法在检测各种科学研究中的罕见差异方面具有更广泛的适用性.