微塑料积累了优先的抗生素耐药性病原体:来自河流塑层的证据
Isabel Silva1, Elsa T Rodrigues2, Marta Tacão3
1Department of Life Sciences, Centre for Functional Ecology, Associate Laboratory TERRA, Faculty of Sciences and Technology, University of Coimbra, 3000-456, Coimbra, Portugal; CESAM (Centre for Environmental and Marine Studies) University of Aveiro, 3810-193, Aveiro, Portugal.
Environmental pollution (Barking, Essex : 1987)
|June 11, 2023
概括
河流中的微塑料可以藏和传播抗生素耐药细菌 (ARB),包括危险的病原体. 污水处理厂的排放对这些微塑料上发现的类型和抗性基因有显著的影响.
科学领域:
- 环境微生物学 环境微生物学
- 水生生态学 水生生态学
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 微塑料 (MP) 是水生系统中新兴的环境污染物.
- 国会议员可能会充当微生物群落的载体,包括抗生素耐药细菌 (ARB).
- 了解国会议员在ARB和抗生素耐药性基因 (ARG) 传播中的作用,对于公共卫生和环境安全至关重要.
研究的目的:
- 确定在河水中的MPs上的生物膜中可培养的抗西普罗夫洛克萨辛和抗塞福他辛细菌的丰富性和多样性.
- 描述殖民国会议员的优先病原体,并确定其抗生素耐药性机制.
- 调查MP类型和接近废水处理厂 (WWTP) 排放对河流塑层抵抗力的影响.
主要方法:
- 在MP (聚烯,聚乙烯,聚乙烯二甲) 和沙子上的生物膜被化在河水中.
- 能够培养的抗西普罗夫洛克萨和塞福塔キシ姆的细菌被列举并确定.
- 优先病原体的特征是毒性因子,ARG和耐药性基因转移.
- 基因组分析包括检测intI1,等离子体介导的类素抗性基因,以及 gyrA 和 parC 的突变.
主要成果:
- 在国会议员身上ARB的丰度通常高于在沙子上.
- PP,PE和PET的混合物支持比单独的PP和PET更高的ARB数量.
- 肠杆菌,包括携带ARG的大肠杆菌和肺炎,如blaCTX-M和类抗性基因,普遍存在,特别是在WWTP的下游.
- 许多分离物体表现出多药耐药性,毒性特征和转移耐药基因的能力.
结论:
- 在河流环境中,MPs有助于传播具有临床ARG和毒性特征的多药耐药肠道细菌.
- 庞大物质和WWTP排放的类型是塑造河流塑层电阻体的关键因素.
- 这凸显了MPs作为水生生态系统抗生素耐药性的水库和载体的潜力.
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