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直接通过X射线激光衍射成像的Thermus thermophilus多倍体细胞

Akihiro Suzuki¹, Toshiyuki Moriya², Tairo Oshima²

¹Research Institute for Electronic Science, Hokkaido University.

The Journal of general and applied microbiology

|June 11, 2023

概括

这项研究使用X射线自由电子激光 (XFEL) 衍射直接可视化了Thermus thermophilus中的多重性. 活细胞成像揭示了五个核子排列成一排,证实了细菌细胞内的多个基因组副本.

科学领域:

微生物学微生物学
生物物理学的生物物理.
结构生物学结构生物学

背景情况:

热 (Thermus thermophilus) 是一种众所周知的多体细菌,每细胞具有多个基因组副本.
之前的证据表明T. thermophilus的多体育是基于间接的分子生物学实验.
在活体细菌中直接可视化多体积和内部细胞结构一直是具有挑战性的.

研究的目的:

通过使用活细胞成像,直接检测和可视化Thermus thermophilus中的多粒体.
在适合X射线自由电子激光 (XFEL) 衍射的条件下观察T. thermophilus的内部纳米结构.
建立一种高分辨率的活体细菌细胞成像方法,微米大小的细菌细胞.

主要方法:

开发了一种专门的细菌培养基,以产生较短的T.热细胞 (<2μm).
利用 femtosecond X射线自由电子激光 (XFEL) 脉冲进行高速,无害的活细胞成像.
采用连贯衍射成像与代阶段检索来从衍射模式重建细胞结构.

主要成果:

通过XFEL衍射成功获得了活体T. thermophilus细胞的高分辨率图像.
重建的图像显示了五个不同的峰值,称为核,在单个细胞内线性排列.
观察到的安排证实了T. thermophilus的多体性,其中存在多个基因组副本.

结论:

射线自由电子激光 (XFEL) 衍射为可视化活细菌细胞内部纳米结构提供了一种强大的新方法.
直接成像证实了Thermus thermophilus的多体性质.
这种技术为研究活微生物的细胞组织和基因组组织开辟了道路.

关键词:

热 (Thermus thermophilus) 是一种热的动物.一个X射线自由电子激光.细菌细胞成像细菌细胞成像一致的衍射成像成像.活细胞成像活细胞成像多倍体 prokaryotes 是一个多倍体 prokaryotes.短杆细胞短杆细胞

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