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系统遗传学确定了COVID-19中宿主反应的miRNA介导调节
T Gjorgjieva1,2, A Chaloemtoem3, T Shahin3
1Biology Program, Division of Science, New York University Abu Dhabi, Abu Dhabi, United Arab Emirates. tg1407@nyu.edu.
Human genomics
|June 11, 2023
概括
在COVID-19患者中的微RNA (miRNA) 变异与血液特征和重症监护室 (ICU) 入院有关. 主体遗传学影响miRNA表达,通过转录后调节影响疾病严重程度.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- COVID-19呈现出疾病严重程度的广泛范围.
- 基因表达调节,特别是微RNAs (miRNAs),对于宿主免疫反应和疾病结果至关重要.
- 在COVID-19血液表型和重症监护室 (ICU) 入院中miRNA变化的作用尚不清楚.
研究的目的:
- 调查miRNA表达对COVID-19疾病严重性的贡献.
- 为了确定与血液表型和COVID-19患者的ICU入院相关的特定miRNAs.
主要方法:
- 综合了多omics分析 (基因型,miRNA,RNA表达) 和电子健康记录表型.
- 分析包括阿布扎比的259名未接种疫苗的COVID-19患者.
- 在入院时分析了632个miRNA和62个临床变量.
主要成果:
- 97个miRNA与8个与ICU入院相关的血液表型相关.
- 综合性分析揭示了miRNA-mRNA-血液内类型的关联,包括miR-143-3p通过BCL2对中性粒细胞数的影响.
- 确定了168个cis-miRNA表达量的特征位点,其中57个涉及与ICU入院或血液表型相关的miRNA.
结论:
- 系统遗传学的方法阐明了全血小RNA在未接种疫苗的COVID-19患者中的作用.
- 由miRNAs进行的转录后调节是影响与COVID-19严重程度相关的血液特征的潜在机制.
- 在COVID-19早期阶段,宿主对miRNA表达的遗传控制是显著的.
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