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使用竞争性内源性RNAs网络识别与急性淋巴细胞白血病进展相关的lncRNAs

Shahram Nekoeian¹, Tahereh Rostami², Amir Norouzy³

¹Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Oncology research

|June 12, 2023

概括

像MCM3AP-AS1和IRF1-AS1这样的长非编码RNA (lncRNAs) 是急性淋巴细胞白血病 (ALL) 进展的关键参与者. 这些lncRNAs表现高,并且与癌症途径有关,这表明它们有可能成为ALL的诊断和治疗点.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

急性淋巴细胞白血病 (ALL) 是一种骨髓恶性瘤,其进展原因不明.
有效的治疗方法存在,但需要预后生物标志物用于早期诊断和改善治疗.
长非编码RNAs (lncRNAs) 越来越多地被认为是它们在癌症发展中的作用.

研究的目的:

使用竞争性内源性RNA (ceRNA) 网络识别参与ALL进展的特定lncRNA.
探索这些已识别的lncRNAs作为ALL的新生物标志物的潜力.
阐明候选 lncRNAs 在 ALL 发病过程中的功能性作用.

主要方法:

重新分析GSE67684数据集,以确定ALL中差异表达的lncRNA和mRNA.
使用Targetscan,miRTarBase和miRcode数据库构建一个ceRNA网络,以链接lncRNA,microRNA和mRNA.
使用逆转录定量实时PCR (RT-qPCR) 验证候选 lncRNA 表达.

主要成果:

该ceRNA网络确定了IRF1-AS1,MCM3AP-AS1,TRAF3IP2-AS1,HOTAIRM1,CRNDE和TUG1作为与ALL进展相关的顶级lncRNA.
亚网络分析显示MCM3AP-AS1,TRAF3IP2-AS1和IRF1-AS1与炎症,转移和扩散途径有显著联系.
与对照组相比,在ALL样本中观察到IRF1-AS1,MCM3AP-AS1,TRAF3IP2-AS1,CRNDE和TUG1的表达升高.

结论:

在ALL进展过程中,MCM3AP-AS1,TRAF3IP2-AS1和IRF1-AS1的表达显著增加,这表明其具有瘤性作用.
这些失调的lncRNAs与关键的癌症相关途径有关.
在这项研究中发现的lncRNAs代表了急性淋巴细胞白血病的有前途的治疗和诊断标.

关键词:

所有的,所有的,所有的.在白血病中,白血病.没有编码的RNAs.RT-qPCRR 是一个人.这是一个微型RNA.

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