相关实验视频

Updated: Jul 27, 2025

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

Published on: June 11, 2016

细胞RNA水平定义了活性污泥微生物群落中的异质基质吸收率子集团

Bing Guo^1,2, Dominic Frigon¹

¹Department of Civil Engineering and Applied Mechanics, McGill University, 817 Sherbrooke Street West, Montreal, Quebec, Canada H3A 0C3.

Interface focus

|June 12, 2023

概括

污水处理细菌根据它们的消耗容易或缓慢降解的基板 (RDS/SDS) 来分类. RNA和多基酸盐 (PHA) 水平有效地在活性污泥社区中识别了这些细菌群体.

科学领域:

环境微生物学环境微生物学
生物技术是生物技术.
废水处理过程废水处理过程

背景情况:

一个以前提出的异质型专家模型将废水处理厂 (WWTP) 异质型生物分为易降解基质 (RDS) 和缓慢降解基质 (SDS) 消费者.
代谢建模预测了RNA和多基酸盐 (PHA) 水平之间的正相关性,高水平表明RDS消费者,低水平表明SDS消费者.

研究的目的:

为了验证RNA和PHA水平作为RDS和SDS消费者分工会在活性污泥中的生物标志物的预测.
基于这些生物标志物,利用流细胞计进行细胞分类,随后分析微生物群落.

主要方法:

从三个WWTP收集了样本.
使用RNA和PHA水平作为细胞分类生物标志物的流细胞计.
在经过排序的微生物群体上进行了16S rRNA基因扩增序列测序.
分析了生态生理学特征和移民率,使用了遗传学和大规模流动模型.

主要成果:

在RDS和SDS消费者分支机构之间成功区分了RNA和PHA水平,证实了之前的预测.
经过排序的微生物群体在时间和WWTP之间显示出很高的相似性,基于RNA水平的明显分离.
遗传学分析表明,高RNA种群具有RDS消费者的特征,例如较高的rRNA基因拷贝数量.
在高RNA种群中,移民率更高,特别是在较低的固体居住时间.

结论:

RNA和PHA水平是可靠的生物标志物,用于区分WWTP活性污泥中的RDS和SDS消费者.
这些子集团的组成在WWTP中相对稳定,并受固体停留时间等操作参数的影响.

关键词:

活性污泥活动污泥这是一个决定性因素.流动细胞计量是流动细胞计量的方法.光激活细胞分类光激活细胞分类异构的工会异构的工会微生物社区的微生物社区.

更多相关视频

Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli

Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli

Published on: August 22, 2017

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments

Published on: October 2, 2012

相关概念视频

Bacterial RNA Polymerase

Bacterial RNA Polymerase

Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...

Stringent Response in E. coli

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...

Translational Regulation

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...

Types of RNA

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

Transcriptional Regulation: Riboswitches

Transcriptional Regulation: Riboswitches

Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...