一种新的lncRNA DFRV在流感A病毒感染中起着双重作用
Keyu Wang1, Meiliang Gong1, Sumin Zhao2
1Department of Clinical Laboratory, National Clinical Research Center for Geriatric Diseases, The Second Medical Center of Chinese PLA General Hospital, Beijing, China.
Frontiers in microbiology
|June 12, 2023
概括
一种新的长非编码RNA,双重功能调节流感病毒 (DFRV) 被确定. 在A型流感病毒感染中,DFRV充当双重调节剂,影响病毒复制和先天免疫反应.
科学领域:
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
背景情况:
- 长非编码RNA (lncRNAs) 在生物活动中发挥作用,包括免疫反应.
- 在抗病毒先天免疫中 lncRNAs 的特定功能在很大程度上仍未被描述.
研究的目的:
- 识别和描述参与对流感A病毒 (IAV) 感染的先天免疫反应的新型lncRNAs.
- 阐明这些lncRNAs在病毒感染和宿主防御中的调节机制.
主要方法:
- 在IAV感染期间识别新型lncRNA,DFRV.
- 对DFRV表达模式和对NFκB信号通路的依赖性的分析.
- 研究DFRV拼接成两个功能转录 (DFRV长DFRV短).
- 评估DFRV对病毒复制和促炎性细胞因子 (IL-1β,TNF-α) 生产的影响.
- 检查DFRV调节的信号通路,包括NFκB,STAT3,PI3K/AKT和MAPK通路.
- 评估DFRV短距离和DFRV长距离之间的相互作用.
主要成果:
- 在IAV感染期间,DFRV表达以剂量和时间依赖的方式增加,由NFκB途径介导.
- DFRV被分割成两个转录:长DFRV,抑制病毒复制,短DFRV,促进病毒复制.
- DFRV通过激活促炎信号级联 (NFκB,STAT3,PI3K/AKT,ERK1/2,p38) 来调节IL-1β和TNF-α的产生.
- 短DFRV可以抑制长DFRV的表达.
结论:
- 在IAV感染中,DFRV充当了一种新型的双重调节剂.
- DFRV有助于病毒复制和先天免疫反应之间的复杂相互作用.
- DFRV的双重功能突出了其在病毒感染期间维持先天免疫恒温的潜在作用.
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