在植物植物生长周期期间,草贝戈莫病毒物种多样性发生变化
Anna E Dye1, Brenda Muga2, Jenniffer Mwangi2
1Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, United States.
Frontiers in microbiology
|June 12, 2023
概括
香草马赛克病 (CMD) 的动态因传播而有所不同. 东非木马赛克病毒 (EACMV) 在温室传播中占主导地位,而只有非洲木马赛克病毒 (ACMV) 是白传播的,影响了木生产.
科学领域:
- 植物病理学 植物病理学
- 病毒学 病毒学
- 分子生物学分子生物学
背景情况:
- 大麻 (Manihot esculenta) 是非洲重要的粮食安全作物,受到大麻马赛克病 (CMD) 的威胁.
- 冠状病毒疾病是由贝戈莫病毒 (Geminiviridae家族) 引起的,由甜白 (Bemisia tabaci) 传播.
- 了解不同草马赛克贝戈莫病毒 (CMB) 种类的流行和传播动态对于疾病管理至关重要.
研究的目的:
- 在不同传播条件下调查肯尼亚不同CMB物种的动态.
- 为了比较病毒在植物性传播后的流行和主导地位与白传播.
主要方法:
- 在肯尼亚 (维多利亚湖和沿海地区) 对CMD受影响的麻实地采样.
- 温室繁殖和维护样本中的麻瓜植物.
- 在银河平台上进行下一代测序 (Illumina) 和生物信息分析.
- 用特定的贝戈莫病毒进行白媒介传播实验.
主要成果:
- 现场样本显示出多种CMB,包括ACMV,EACMV,EACMKV,EACMV-Ug和EACMZV,频繁出现混合感染.
- 经过3年的温室植物传播,EACMV类病毒在所有样本中占主导地位.
- 白传播实验表明,与EACMV类病毒不同,只有ACMV从混合感染中有效传播.
结论:
- 在温室中进行植物性繁殖有利于EACMV类病毒的主导地位.
- 白传播动态与ACMV和EACMV类病毒的植物性传播动态有很大不同.
- 这些发现突出了影响CMB演变和麻豆疾病传播的独特传播结果.
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