病毒颗粒和非外病毒样颗粒的尺寸选择性下游加工
Nils Hillebrandt1, Jürgen Hubbuch1
1Institute of Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
Frontiers in bioengineering and biotechnology
|June 12, 2023
概括
尺寸选择性分离技术为净化非外病毒样颗粒 (VLP) 提供了一个有前途的解决方案. 这些方法利用尺寸差异有效地去除杂质,增强生物制药应用.
科学领域:
- 生物技术是生物技术.
- 蛋白质纳米粒子工程 工程
- 生物加工是一种生物加工.
背景情况:
- 非外病毒样颗粒 (VLP) 是具有显著生物制药潜力的蛋白质纳米颗粒.
- 传统的下游处理 (DSP) 方法由于其大小而面临VLP的挑战.
- 主体细胞杂质往往使VLP净化复杂化.
研究的目的:
- 审查VLP净化大小选择性分离技术的原则和应用.
- 突出这些技术在病毒颗粒 (VPs) 下游处理中的潜力.
- 讨论非封装VLP及其子单元的特定DSP步骤.
主要方法:
- 对尺寸选择性分离原则的审查.
- 对VLP现有的DSP技术的分析.
- 探索基于大小的净化策略.
主要成果:
- 尺寸选择性分离有效地利用了VP和杂质之间的尺寸差异.
- 这些技术在各种VP中显示出广泛的适用性.
- 在VLP生产中提高纯度和产量的潜力.
结论:
- 尺寸选择性分离是VLP下游加工的一个有价值的方法.
- 这些方法为生物制药应用提供了可扩展和多功能解决方案.
- 进一步开发可以优化VLP净化效率.
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