二倍体草Aegilopscomosa的物种内分歧与结构性染色体变化有关
Ekaterina D Badaeva1,2, Violetta V Kotseruba3, Andnrey V Fisenko1
1N.I.Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkina str. 3, GSP-1, Moscow 119991, Russia Engelhardt Institute of Molecular Biology, Russian Academy of Sciences Moscow Russia.
Comparative cytogenetics
|June 12, 2023
概括
由于染色体变化,包括转位和改变的DNA分布,Aegilops comosa亚种分离. 这种遗传变异,可能是由开放授粉驱动的,在这种双胞胎草中创造了广泛的物种内部多样性.
科学领域:
- * 植物学 植物学
- * 遗传学 在遗传学方面
- * 分子生物学 * 分子生物学
背景情况:
- * Aegilops comosa 是一种来自希腊的双胞胎草 (MM基因组).
- * 两个亚种,AE. c. 科莫萨和艾. c. heldreichii,是公认的,但它们的遗传差异尚不清楚.
- * 了解亚种分歧对于植物遗传多样性研究至关重要.
研究的目的:
- *为了描述Aegilops comosa的基因组和型.
- * 评估基因多样性和亚种辐射的机制.
- * 调查AE之间分歧的遗传和刻型基础. c. 科莫萨和艾. c. 举行reichii. 的时间.
主要方法:
- *使用重复性DNA探针进行光在位杂交 (FISH).
- * 格里亚丁的电泳分析.
- *对3M和6M染色体进行型分析.
主要成果:
- *亚种在染色体大小/形态 (3M,6M) 上有所不同,这表明相互转移.
- *观察到微卫星/卫星DNA分布和NOR位置的变化.
- * Gliadin光谱,特别是在a区,显示了亚种特定的差异.
- *杂交物很常见,有助于广泛的物种内部变异.
结论:
- *染色体重新排列和DNA序列变异是Aegilops comosa的亚种分歧的基础.
- * 开放式授粉和缺乏障碍物有助于广泛的物种内部变异.
- * 发现突出了Aegilops comosa中显著的遗传异质性.
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