乳酸对突纤维细胞和巨细胞效应体功能的差异作用
Valentina Pucino1,2, Meriam Nefla1, Vincent Gauthier1,2
1Rheumatology Research Group, Institute of Inflammation and Ageing, University of Birmingham, Birmingham, United Kingdom.
Frontiers in immunology
|June 12, 2023
概括
风湿性关节炎 (RA) 关节中的高乳酸水平独特地影响纤维细胞和巨细胞的功能. 乳酸盐促进纤维细胞活性,同时抑制巨细胞,为RA提供新的治疗点.
科学领域:
- 类风湿病学 类风湿病学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 类风湿性关节炎 (RA) 涉及突膜的炎症.
- 关节炎综合体的特征是缺氧,酸性和乳酸水平升高.
- 已经确定了不同的纤维细胞和巨细胞子集,它们在RA病变发生过程中发挥了特定的作用.
研究的目的:
- 研究乳酸对纤维细胞和巨细胞运动,IL-6分泌和新陈代谢的调节作用.
- 探索特定乳酸运输体 (SLC16A1和SLC16A3) 在RA突细胞中的作用.
主要方法:
- 来自RA患者和对照组的突组织分析.
- 免疫光染和共聚焦显微镜以评估乳酸转运体表达.
- 在实验室中使用RA同胞纤维细胞和单细胞衍生的巨细胞进行研究,以评估对乳酸的反应中的迁移,新陈代谢和IL-6分泌.
主要成果:
- 乳酸转运体SLC16A1和SLC16A3在RA突组织中表达,并在炎症期间升高调节,在纤维细胞和巨细胞中具有差异表达.
- 乳酸盐在生理性RA度 (10mM) 时会对细胞功能产生差异性影响:它促进纤维细胞迁移,IL-6产生和糖解.
- 相反,乳酸减少了葡萄糖分解,迁移和巨细胞中的IL-6分泌.
结论:
- 这项研究首次证实了纤维细胞和巨细胞在RA关节中对高乳酸水平的明显功能反应.
- 这些发现为RA的发病性提供了新的见解.
- 乳酸酸所确定的差异性作用为RA提供了潜在的新型治疗点.
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