使用两个不同的核酸测试 (NAT) 平台对输血可传播病毒感染的献血血查:一个单一的三级医疗瘤中心体验中心

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Updated: Jul 27, 2025

A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction

A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction

Published on: April 5, 2018

Amardeep Pathak¹, Devasis Panda², Manushri Sharma³

¹Department of Transfusion Medicine, Rajiv Gandhi Cancer Institute and Research Center, Rohini, Delhi India.

Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion

|June 12, 2023

概括

核酸检测 (NAT) 通过对输血可传播感染的查,提高了血液的安全性. 与Procleix Utrio Plus TMA-ID-NAT相比,Cobas® MPX2 PCR-MP-NAT在检测血清学错过的感染方面表现出更高的收益率.

科学领域:

输血安全输血安全
传染病查传染病查
分子诊断学分子诊断

背景情况:

核酸检测 (NAT) 对于在捐献血液中查输血传染性感染 (TTIs) 至关重要.
仅仅通过血清检测,可能会错过早期或低水平的感染.
评估不同的NAT平台对于优化血液安全协议至关重要.

研究的目的:

为了比较两个NAT格式的性能:cobas® MPX2 PCR-minipool NAT (MP-NAT) 和Procleix Utrio Plus TMA-个人捐赠者-NAT (ID-NAT) 的性能.
评估每个NAT方法对病毒TTIs的收益率和检测能力.
评估cobas® MPX2 PCR-MP-NAT在印度血液查中的适用性.

主要方法:

70个月的血液银行数据的回顾性分析.
通过使用cobas® MPX2 PCR-MP-NAT和Procleix Utrio Plus TMA-ID-NAT对48,151个捐赠者的查.
将NAT结果与初始血清学和快速卡检测查对HIV,HBV,HCV,梅毒和疟疾进行比较.

主要成果:

MP-NAT的整体NAT收益率为1:281%,而ID-NAT的收益率为1:3242.
MP-NAT检测到13例HBV和1例HCV感染被血清学遗漏,而ID-NAT检测到5例HBV感染被血清学遗漏.
与ID-NAT (34.6%) 相比,MP-NAT显示了较高的血清活性捐赠比例 (59.8%).

结论:

科巴斯® MPX2 PCR-MP-NAT显示了较高的整体NAT产率,并检测出更多的感染错过了血清学比Procleix Utrio Plus TMA-ID-NAT.
cobas® MPX2 PCR-MP-NAT系统,其操作方便和简单的算法,是印度血液查的潜在有效解决方案.
使用NAT进行增强查,通过检测传统方法错过的感染,显著提高了血液安全.

关键词:

迷你游泳池迷你游泳池迷你游泳池在 NATAT NAT 中使用.输血输血输血是指输血.传染性感染是一种传染性感染.

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