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Updated: Jun 9, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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基于TMT的最小数量的细胞的蛋白质组分析的一阶段尖端方法

Narae Park1,2, Hankyul Lee1,2, Yumi Kwon1

  • 1Chemical & Biological Integrative Research Center, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea.

ACS omega
|June 12, 2023
PubMed
概括

我们开发了OnM,这是一种用于分析有限的生物样本的新型蛋白质组学方法. 这种技术最大限度地减少了样本损失,使得从50个细胞中有效量化蛋白质.

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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...