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在心血管血栓性中风中识别和交叉验证与自相关的基因
Yufang Yang1, Min Zhang2, Ziqing Li1
1School of Basic Medical Sciences, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Frontiers in neurology
|June 12, 2023
概括
心血管血栓性中风 (CE中风) 涉及自. 生物信息学确定了41个与自相关的基因,包括SERPINA1和CXCR4,为CE中风治疗提供了潜在的治疗标.
科学领域:
- 生物医学研究的研究.
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 心血管栓塞性中风 (CE中风) 是一种严重的缺血性中风亚型,复发率和死亡率很高.
- 脑脊髓中风的潜在病原体尚未完全理解,尽管自也与此有关.
- 识别分子标记物和治疗点对于改善CE中风结果至关重要.
研究的目的:
- 使用生物信息学识别CE中风的潜在自相关分子标记物.
- 根据与自相关的基因表达,发现CE中风的潜在治疗点.
- 阐明自在CE中风的发展中的作用.
主要方法:
- 使用来自 GEO 数据库的 GSE58294 mRNA 表达式数据集.
- 使用R软件在CE中风中选与自相关的差异表达 (DE) 基因.
- 进行了蛋白质-蛋白质相互作用 (PPI),相关性和基因本体学 (GO) 丰富分析.
- 使用数据集GSE66724,GSE41177和GSE22255与学生的t测试验证了DE基因.
主要成果:
- 在CE中风患者和健康对照者之间确定了41个与自相关的DE基因 (37个上调,4个下调).
- GO和KEGG丰富分析揭示了与自,亡和ER压力相关的术语.
- 发现了枢纽基因,包括CXCR4 (一般性中风) 和特定的CE中风基因,如ARNT,MAPK1,ATG12,ATG16L2,ATG2B和BECN1.
结论:
- 确定了41个与自相关的基因作为CE中风的潜在生物标志物.
- 塞尔皮纳1,WDFY3,ERN1,RHEB和BCL2L1是重要的DE基因,可能通过自性调节CE中风.
- 这些发现提供了关于自在CE中风中的作用的见解,并建议新的治疗点.
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