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使用色带和45S rDNA-FISH进行八种Cucurbitaceae作物的比较型分析

Chao-Wen She^1,2, Xiang-Hui Jiang¹, Chun-Ping He²

¹Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, Huaihua University, Huaihua, Hunan, 418008, China Huaihua University Huaihua China.

Comparative cytogenetics

|June 12, 2023

概括

在使用染色体带和FISH分析了八种Cucurbitaceae作物的型变异. 这项研究为精确的染色体识别和理解家族内的基因组分化奠定了基础.

科学领域:

植物遗传学和基因组学
细胞遗传学和分子细胞遗传学

背景情况:

型分析对于理解植物的基因组进化和物种关系至关重要.
黄瓜科家族表现出多样化的形态,需要详细的细胞遗传研究.

研究的目的:

在八种Cucurbitaceae物种中调查型变异和 heterochromatin 和 45S rDNA 的染色体分布.
建立详细的型并阐明这些物种之间的型关系.
讨论基因组差异化基于型数据和遗传学关系.

主要方法:

用于染色体制备的酶和火焰干燥.
组合PI和DAPI (CPD) 染色用于异色染色素分析.
用45S rDNA探针进行光在位杂交 (FISH).
型不对称指数 (CV_CI,CV_CL,M_CA) 和主要坐标分析 (PCoA).

主要成果:

所有研究的物种都表现出对称的型,可以通过M_CA和CV_CL散射图区分.
理学关系通常与基于DNA的遗传学关系一致.
CPD染色揭示了异染色质的模式,而FISH确定了45SrDNA位点,其数量和位置在物种之间有所不同.
黄瓜 (Cucumis sativus) 染色体可以使用CPD带和45SrDNA信号精确识别.

结论:

型分析提供了对基因组分化的洞察力,以及Cucurbitaceae内部的遗传学关系.
特定的带状和rDNA模式在黄瓜等作物物种中提供了精确染色体识别的潜力.
这项研究强调了植物基因组研究中综合细胞遗传方法的实用性.

关键词:

黄瓜科 (Cucurbitaceae) 是一个植物.细胞分类学细胞分类学光在现场混合化光.色带带带的色带带.型是一种型.型的非对称性不对称性核糖体RNA基因 (rDNA) 是一种

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FISH - Fluorescent In-situ Hybridization

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...