抗生素耐药Acinetobacter baumannii中recA和umuDC的基因表达的改变
Nada Ali Abdul Ameer1, Maysaa Abdul Razzaq Dhahi2
1Bacteriology Laboratory, Al-Imamein Al-Kadhimein Medical City, Baghdad, Iraq.
Journal of medicine and life
|June 12, 2023
概括
在Acinetobacter baumannii中,recA和umuDC基因的高表达与抗生素耐药性的增加有关. 这表明SOS响应在开发广泛耐药菌株方面发挥了作用.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 菌 (Acinetobacter baumannii) 是一种重要的病原体,以其在抗生素耐药性方面的重要作用而闻名.
- SOS (拯救我们的船) 系统是一种DNA修复机制,在细菌的生存和适应中起着至关重要的作用,包括在抗生素耐药性方面.
研究的目的:
- 研究recA和umuDC基因的表达水平之间的关联,SOS通路的关键组成部分,和Acinetobacter baumannii的抗生素耐药性.
- 探索SOS基因上调对A. baumannii中广泛耐药 (XDR) 现型的发展的潜在贡献.
主要方法:
- 分析了78种临床和31种生态分离的Acinetobacter baumannii.
- 使用Vitek-2系统进行细菌鉴定和抗生素敏感性测试.
- 使用PCR对blaOXA-51和blaOXA-23基因进行A. baumannii的分子确认.
- 定量实时PCR (qRT-PCR) 用于确定recA和umuDC基因的表达水平.
主要成果:
- 在14/25临床菌株中观察到recA的上调,而7/25菌株显示recA和umuDC的上调.
- 16/25广泛耐药的临床菌株表现出recA和/或umuDC的高表达.
- 在生态菌株中,recA上调可见于3/6,recA和umuDC上调可见于1/6.
结论:
- 在Acinetobacter baumannii菌株中,recA和/或umuDC基因的高表达水平可能有助于增加对广泛抗生素的耐药性.
- 这些SOS基因的上调可能是导致A. baumannii.广泛耐药 (XDR) 现型出现的一个因素.
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