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对两动物 (Xenopus laevis) 髓状细胞子集之间的关系的一种视角

Muhammad Riadul Haque Hossainey¹, Kelsey A Hauser¹, Christina N Garvey¹

¹Department of Biological Sciences, The George Washington University, 800 22nd Street Northwest, Suite 6000, Washington DC 20052, USA.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences

|June 12, 2023

概括

两类巨细胞 (Mφs) 与互白素-34 (IL34) 分化,与树突细胞 (DCs) 有共同特征,与殖民地刺激因子-1 (CSF1) 不同. 这些IL34-Mφs和FLT3L衍生的DCs显示了对Mycobacterium marinum的增强免疫反应.

科学领域:

免疫学免疫学免疫学
发展生物学发展生物学
比较生物学的比较生物学

背景情况:

在脊椎动物中,巨细胞 (Mφ) 的分化和功能由殖民地刺激因子-1 (CSF1) 和互白素-34 (IL34) 细胞因子调节.
两动物Mφs,特别是在*Xenopus laevis*,当与CSF1与IL34.4区分时,表现出明显的特征.
哺乳动物Mφs与树突细胞 (DCs) 具有共同的祖先,它们依赖fms类型的氨酸激酶3连接体 (FLT3L) 来进行分化.

研究的目的:

为了比较 *Xenopus laevis* 的 CSF1-Mφs, IL34-Mφs 和 FLT3L 衍生 DC.
研究这些细胞类型之间的转录特征和功能能力的相似性和差异.
了解Mφ和DC分化路径的进化分歧.

主要方法:

对 *Xenopus laevis* CSF1-Mφs,IL34-Mφs 和 FLT3L-DCs 的转录概况.
功能性检测包括混合白细胞反应 *in vitro*.
评估 *in vivo* 对 *Mycobacterium marinum* 的免疫反应.

主要成果:

IL34-Mφs和FLT3L-DCs表现出显著的转录和功能共同点,使它们与CSF1-Mφs区别开来.
与CSF1-Mφs相比,IL34-Mφs和FLT3L-DCs表现出更高的表面主要基因相容性复合体 (MHC) I类表达.
两种IL34-Mφs和FLT3L-DCs都表现出在引起混合白细胞反应和产生*体内*适应性免疫力对*M. marinum*的优越能力.

结论:

*Xenopus laevis* IL34-Mφs具有与FLT3L-依赖的DC重叠的特征,这表明脊椎动物骨髓形成中的保护和分离的途径.
这些发现提供了关于Mφ和DC分化的演变的见解,突出了不同的功能角色.
对非哺乳动物骨髓形成的进一步研究可以揭示保存和分离的免疫细胞发育途径.

关键词:

两动物的骨髓形成 (Myelopoiesis) 是一种殖民地刺激因子-1-1 树突细胞是一种树突细胞.fms类似的铁氨酸激酶-3连接体.介质蛋白-34 介质蛋白-34 巨细胞是什么?巨细胞是什么?

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