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PARP12 调节了小鼠卵细胞中性成熟的过程

Guangyi Cao^1,2,3, Ruirui Guo^1,2, Manqi Chen^1,2

¹Key Laboratory for Stem Cells and Tissue Engineering (Sun Yat-sen University), Ministry of Education, Guangzhou, China.

Journal of cellular physiology

|June 12, 2023

概括

多分子ADP-ribosyl化 (PARylation) 对于卵细胞成熟至关重要. 这项研究揭示了Parp12,一个单一的 (ADP-ribosyl) 转移酶,对于在小鼠卵细胞化过程中适当的螺丝组织和染色体对齐至关重要.

科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
生殖生物学生殖生物学

背景情况:

聚ADP-ribosyl化 (PARylation) 是一个关键的翻译后修饰,涉及到许多细胞过程.
虽然PARylation在卵细胞成熟中的作用已得到认可,但Mono(ADP-ribosyl) 化 (MARylation) 的特定贡献在很大程度上仍未被探索.

研究的目的:

为了研究Parp12的作用,一个Marylating酶,在小鼠卵细胞中介成熟.
阐明Parp12影响卵细胞发育的分子机制.

主要方法:

在卵细胞成熟过程中对Parp12表达和局部化的定量分析.
功能性研究涉及小鼠卵细胞中Parp12耗尽 (敲击) 的研究.
评估螺旋组织,染色体对齐和形积分.
对螺旋组装检查点激活和F-actin动态的分析.
转录组分析以评估全球基因表达变化.

主要成果:

在整个卵细胞成熟过程中,Parp12的表达很高,并且表现出动态局部化,包括在杆附近的聚合.
帕尔p12的耗尽导致异常的螺旋组织,染色体不对齐,增加了体积.
帕普12敲击激活了螺旋组装检查点,并降低了F-actin水平.
转录组分析显示,Parp12的枯竭破坏了整个转录组的恒常性.

结论:

孕产妇表达的Parp12对于保持基因组稳定性和小鼠卵细胞化正常进展至关重要.
在卵细胞成熟过程中,Parp12在调节组织,染色体分离和潜在的不对称分裂方面发挥着至关重要的作用.

关键词:

马里化 (MARylation) 是一种在PARP12中,PARP12是PARP12中的一个.介质变化 (meiosis) 是一种变质的过程.卵子细胞卵子细胞生殖生殖的繁殖.

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