Y复合核波林独立地对神经元前代的核孔组合和基因调节作出贡献
Clarisse Orniacki1, Annalisa Verrico1, Stéphane Pelletier1
1Université Paris Cité, CNRS, Institut Jacques Monod, F-75013 Paris, France.
Journal of cell science
|June 12, 2023
概括
Y-复杂核波林,Nup133和Seh1,在神经外皮分化过程中调节基因表达. 这一功能独立于核毛孔篮的完整性,突出了在发育基因控制中的共同作用.
科学领域:
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
- 遗传学 遗传学 是一个
背景情况:
- 核素 (Nups) 是核毛孔复合物的关键组成部分,对于核运输至关重要.
- 除了运输之外,Nups在发育过程中在染色质组织和基因表达中发挥着关键作用.
- 作为Y复合体的一部分,Nup133和Seh1对于神经外皮分化的生存至关重要.
研究的目的:
- 研究Nup133在神经外皮分化过程中调节基因表达中的作用.
- 为了确定Y复合核素的基因调节功能是否与核孔篮完整性有关.
- 探索Nup133和Seh1在神经前代基因调节中的共同功能.
主要方法:
- 对Nup133缺乏和野生类型细胞的转录组分析.
- 在Nup133和Seh1缺陷的祖先中分析基因表达 (Lhx1,Nup210l).
- 核孔复合结构的评估,特别是篮子组装.
主要成果:
- 在早期神经外皮分化过程中,Nup133调节特定的基因,包括Lhx1和Nup210l.
- 错误的Lhx1和Nup210l的调节发生在Nup133ΔMid的祖先中,他们的篮子组装受损.
- 减少Nup133水平或轻微减少Seh1缺乏细胞的孔密度,也会错误调节这些基因,独立于篮子完整性.
结论:
- 在神经外皮分化过程中,Y复合核波林 (Nup133,Seh1) 在调节基因表达方面具有共同的功能.
- 这种基因调节作用似乎独立于核孔篮的结构完整性.
- Nup133和Seh1通过与它们在核孔支架中的作用不同的机制,对发育基因控制做出贡献.
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