相关实验视频

Updated: Jul 26, 2025

Detection of Plasmodium Sporozoites in Anopheles Mosquitoes using an Enzyme-linked Immunosorbent Assay

Detection of Plasmodium Sporozoites in Anopheles Mosquitoes using an Enzyme-linked Immunosorbent Assay

Published on: September 30, 2021

杆菌TRAP的形状变化对于虫虫的迁移和传播至关重要

Friedrich Braumann¹, Dennis Klug^1,2, Jessica Kehrer^1,3

¹Integrative Parasitology, Center for Infectious Diseases, Heidelberg University Medical School, Heidelberg, Germany.

|June 12, 2023

概括

粘附于TRAP的Plasmodium sporozoite的形状变化对于滑翔运动性和传播至关重要. 稳定TRAP的I域影响寄生虫的运动和传染性.

科学领域:

寄生虫学的寄生虫学
细胞生物学细胞生物学
结构生物学结构生物学

背景情况:

细胞的粘附和迁移取决于表面粘附素,这些粘附素将细胞外连接物连接到细胞内actin细胞骨架.
在蚊子传播后,Plasmodium sporozoites利用粘附和滑动运动来殖民唾液腺和入侵宿主肝脏.

研究的目的:

为了研究TRAP的插入 (I) 域的Plasmodium sporozoite粘合物的两个构造状态 (开放和关闭) 的功能重要性.
确定这些形状的稳定如何影响杂虫细胞的功能和传播.

主要方法:

产生了表达TRAP的Plasmodium寄生虫,其I域以使用二硫化键稳定在开放或封闭的构造中.
评估了这些稳定TRAP变异对杂虫虫滑动性,蚊子唾液腺进入和传播效率的影响.
研究了降解剂对表达开放的TRAP I域的脊髓体滑翔机动性的影响.

主要成果:

稳定TRAP I域在开放或封闭的构造中显著损害了 sporozoite 滑动,唾液腺进入和传播.
在被减少剂治疗时,表达开放的TRAP I域的寄生虫中部分恢复了sp功能,这表明了动态形状变化的作用.
这项研究强调了TRAP的结构动态在重要寄生虫功能中的关键作用.

结论:

在TRAP I域中的动态形状变化对于Plasmodium sporozoite连接体结合,滑翔运动性和器官入侵至关重要.
这些形状动态对于成功从蚊子传播到哺乳动物至关重要.

关键词:

粘附在其中的粘合物.细胞迁移细胞迁移滑翔机动性的滑翔机动性整合物是一种整合物.疟疾疟疾是一种疾病.

更多相关视频

Plasmodium falciparum Gametocyte Culture and Mosquito Infection Through Artificial Membrane Feeding

Plasmodium falciparum Gametocyte Culture and Mosquito Infection Through Artificial Membrane Feeding

Published on: July 3, 2020

Generating Genetically Modified Plasmodium berghei Sporozoites

Generating Genetically Modified Plasmodium berghei Sporozoites

Published on: May 5, 2023

相关概念视频

Symbiosis

Symbiosis

Symbiotic relationships are long-term, close interactions between individuals of different species that affect the distribution and abundance of those species. When a relationship is beneficial to both species, this is called mutualism. When the relationship is beneficial to one species but neither beneficial nor harmful to the other species, this is called commensalism. When one organism is harmed to benefit another, the relationship is known as parasitism. These types of relationships often...

Diversity of Protists II

Diversity of Protists II

Alveolates are a group of organisms recognized by the presence of alveoli, which are cytoplasmic sacs located beneath the cell membrane. While their function remains uncertain, alveoli may help regulate water balance by controlling how much water enters and leaves the cell. In dinoflagellates, these structures may serve as armor plates. There are three major types of alveolates: ciliates, which move using cilia; dinoflagellates, which use flagella for movement; and apicomplexans, which are...

Mechanism of Conjugation

Mechanism of Conjugation

Bacterial conjugation is a mechanism of horizontal gene transfer that enables the exchange of genetic material between bacterial cells through direct contact. This process is facilitated by a donor cell carrying a conjugative plasmid, which encodes genes necessary for pilus formation, DNA replication, and transfer. The conjugative plasmid plays a central role in initiating and executing the transfer of genetic material.The tra region of the conjugative plasmid encodes proteins responsible for...

Diversity of Protists IV

Diversity of Protists IV

Amoebozoa represent a diverse group of terrestrial and aquatic protists that utilize lobe-shaped pseudopodia for locomotion and feeding. This characteristic differentiates them from the Rhizaria, which possess threadlike pseudopodia. The primary classifications within Amoebozoa include gymnamoebas, entamoebas, and the plasmodial and cellular slime molds. Phylogenetic evidence indicates that Amoebozoa diverged from a lineage that ultimately gave rise to fungi and animals.Gymnamoebas and...

Fungal Phylum Microsporidia

Fungal Phylum Microsporidia

Microsporidia are a group of obligate intracellular fungi that were initially classified as protists but were later reclassified based on phylogenetic, molecular, and structural evidence linking them to the Chytridiomycota. These unicellular, non-motile organisms are highly specialized parasites that infect a wide range of animal hosts, including humans. They have evolved extensive genomic and metabolic reductions, making them highly dependent on their hosts for survival.Morphology and Genomic...

Cotranslational Protein Translocation

Cotranslational Protein Translocation

Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...