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在玉米中结合多重基因编辑和双倍倍基因技术

Lennert Impens^1,2, Christian D Lorenzo^1,2, Wout Vandeputte^1,2

¹Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052, Ghent, Belgium.

The New phytologist

|June 12, 2023

概括

研究人员开发了一种用于玉米的快速CRISPR/Cas9基因编辑方法,为研究基因功能和改进叶子大小等作物特征创造了同卵性突变物.

科学领域:

植物遗传学和分子生物学
农业生物技术农业生物技术
基因编辑技术基因编辑技术

背景情况:

多重CRISPR/Cas9基因编辑允许同时针对多个基因,但获得功能分析的同卵性突变体是劳动密集的.
主要转变体经常表现出异质等位基因突变或遗传马赛克,需要广泛的繁殖和基因造型.

研究的目的:

开发一种快速有效的策略,通过各种组合的同卵性编辑来生成基因相同的植物系.
为了促进基因家族的研究,并确定有益的等位基因组合,以改善作物.

主要方法:

在Zea mays (玉米) 中结合高度多重的CRISPR/Cas9基因编辑与体内平分体诱导.
通过胚胎救援加倍利用高效的双倍的单体植物体外生成.
采用了三种CRISPR/Cas9结构,针对36个参与叶子生长的基因.

主要成果:

在三代内产生了一系列同卵性突变系,具有多种编辑组合.
确定了几种基因型,包括一个七重突变,表现出可再生的叶子大小增加10%.
证明了组合基因编辑和双倍倍基因策略的效率.

结论:

开发的策略显著加速了用于植物功能基因组学的更高阶同卵性突变体的产生.
这种方法对研究基因家族和发现增强农作物定量特征的等位基因组合有价值.
促进高通量表型化,并加速改良作物品种的育种计划.

关键词:

这就是CRISPR/Cas9的作用.一个双倍的平分体.基因编辑基因编辑基因家族基因家族基因家族随机诱导的哈普洛伊德诱导玉米玉米玉米是一种多重基因编辑多重基因编辑突变堆叠是指突变堆叠.

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