超越DNA:用于PAINT超分辨率显微镜的新探针
Marrit M E Tholen1, Roderick P Tas2,3, Yuyang Wang3,4
1Department of Biomedical Engineering, Institute of Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, The Netherlands. l.albertazzi@tue.nl.
Chemical communications (Cambridge, England)
|June 12, 2023
概括
在纳米级地形绘制 (PAINT) 中成像的点积累是单分子定位显微镜 (SMLM) 技术的关键. 本综述探讨了新兴的无DNAPAINT探针,将SMLM应用扩展到传统的基于DNA的方法之外.
科学领域:
- 生物物理学的生物物理.
- 纳米技术纳米技术
- 显微镜的使用方法
背景情况:
- 纳米级拓图像的点积累 (PAINT) 是一种强大的单分子定位显微镜 (SMLM) 技术.
- 目前的DNA-PAINT方法依赖于分子成像的短暂DNA相互作用.
- 对于更广泛的SMLM应用,对独立于DNA的PAINT探针的需求越来越大.
研究的目的:
- 审查SMLM的非基于DNA的PAINT探针.
- 讨论这些新型探测器的应用和挑战.
- 为了突出PAINT工具箱的进步.
主要方法:
- 关于PAINT探针的科学出版物的文献综述.
- 基于其结合机制的非DNAPAINT探头的分类.
- 报告的应用和限制的分析.
主要成果:
- 确定了四个主要类别的无DNAPAINT探头:内源相互作用,工程结合剂,融合蛋白和合成分子.
- 在SMLM中展示了这些探针的各种应用.
- 概述了非DNAPAINT探测器开发的当前挑战和未来方向.
结论:
- 非DNA PAINT 探针显著扩大了 SMLM 的多功能性和适用性.
- 这些替代探头为纳米尺度成像提供了互补的策略.
- 对新型结合机制的进一步研究将增强SMLM的能力.
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