甲的产生由Methanothrix thermoacetophila通过直接的物种间电子转移与Geobacter metallireducens进行
Jinjie Zhou1,2,3,4, Jessica A Smith3,5, Meng Li1,4
1Archaeal Biology Center, Institute for Advanced Study, Shenzhen University , Shenzhen, Guangdong, China.
mBio
|June 12, 2023
概括
甲基细菌在甲排放中发挥着关键作用,可以通过乙酸盐或直接物种间电子转移 (DIET) 产生甲. 这项研究揭示了食期间Metanothrix的潜在电子转移机制,为控制甲生产提供了洞察力.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 生物化学 生化学
背景情况:
- 甲诺特里克斯 (Methanothrix) 是无毒环境中产生甲的关键微生物.
- 它使用了乙类甲基生成和直接的物种间电子转移 (DIET).
- 它的生理学,特别是在饮食期间,还不太清楚.
研究的目的:
- 在使用Geobacter metallireducens的饮食中调查Methanothrix的生理机制.
- 为了确定涉及到饮食中的电子转移通路.
- 了解环境因素如何影响Methanothrix的生长和新陈代谢.
主要方法:
- 对与Geobacter metallireducens共同培养的Methanothrix thermoacetophila进行了转录组分析.
- 使用或不使用磁铁和颗粒活性炭 (GAC) 的种植.
- 分析与电子转移,碳固定和细胞结构相关的基因表达.
主要成果:
- 磁石通过乙烯基聚类甲生成和饮食增强了生长; GAC 损害了生长.
- 关键的Geobacter外膜细胞染色体 (OmaF-OmbF-OmcF,Gmet_0930) 参与了饮食.
- 在不同条件下,Methanothrix显示碳固定和结构基因 (MspA,SqpA) 的高表达.
- 在DIET培养的细胞中,气囊基因表达较低,可能有助于膜接触.
结论:
- 在饮食过程中确定了Geobacter和Methanothrix的潜在电子传递机制.
- 提供了关于无毒环境中Methanothrix生理学的见解.
- 这些发现可以为控制甲排放和优化无氧消化策略提供信息.
相关概念视频
01:15Metabolism of Chemolithotrophs
53
Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.
53
01:28Microbial Nutrition
115
Organisms exhibit remarkable metabolic diversity, categorized based on how they acquire energy and carbon. These strategies enable survival in various ecological niches and are essential for maintaining energy flow and nutrient cycling within ecosystems.Energy and Carbon SourcesOrganisms are classified as phototrophs or chemotrophs based on energy acquisition. Phototrophs use light as their energy source, while chemotrophs rely on oxidizing chemical compounds. Further differentiation arises...
115
01:28Anoxygenic Phototrophic Bacteria
63
Anoxygenic phototrophic bacteria are a diverse group of microorganisms that perform photosynthesis without producing oxygen. They primarily include purple sulfur bacteria, purple nonsulfur bacteria, green sulfur bacteria, and green nonsulfur bacteria. These bacteria are classified into the Gammaproteobacteria, Alphaproteobacteria, Betaproteobacteria, Chlorobi, and Chloroflexi lineages, each with distinct physiological and ecological adaptations.Purple sulfur bacteria belong to the...
63
01:21Hyperthermophilic Bacteria
37
Domain Bacteria includes some unique hyperthermophilic species. They exhibit remarkable adaptations that enable survival in extreme environments.Thermotoga species are rod-shaped, gram-negative, non-sporulating hyperthermophiles that form a sheath-like envelope called a toga. They ferment sugars or starch, producing lactate, acetate, CO₂, and H₂, and can also grow via anaerobic respiration using H₂ and ferric iron. Found in hot springs and hydrothermal vents, over 20% of their...
37
01:29Overview of Archaea
53
Archaea, named after the Archaean eon, represent a unique domain of life, distinct from bacteria and eukaryotes, with remarkable traits. Their cellular and molecular features, ecological adaptability, and industrial relevance highlight their importance in understanding life processes and leveraging biotechnology.Cellular and Molecular CharacteristicsA defining feature of archaea is their unique membrane composition. Archaeal membranes contain ether-linked isoprenoid lipids, which confer...
53
01:28Carbon-dioxide Fixation
43
Carbon dioxide fixation in prokaryotes enables the assimilation of inorganic carbon into organic molecules, supporting biosynthetic pathways, sustaining ecosystems, and contributing to the global carbon cycle. It also has industrial applications in carbon capture and bioproduct synthesis. Autotrophic organisms rely on this process to utilize CO₂ as a carbon source in diverse environments.The Calvin CycleThe Calvin cycle is the most widespread carbon fixation mechanism, primarily used by...
43