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TaRBP1稳定了TaGLTP,并负面调节了小麦的条纹生抗性

Yue Li^1,2, Rongrong Zhang^1,2, Yu Wu^1,2

¹State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China.

Molecular plant pathology

|June 12, 2023

概括

小麦小麦小麦小麦的小麦.

科学领域:

植物病理学植物病理学
分子生物学分子生物学
生物化学生物化学

背景情况:

甲状腺脂质代谢调节植物的防御和编程细胞死亡.
连接脂代谢和植物免疫的分子机制尚未完全理解.

研究的目的:

调查小麦RNA结合蛋白1 (TaRBP1) 在植物防御Puccinia striiformis f. sp. 的作用. 三子 (Pst) 的意思.
阐明TaRBP1在调节植物免疫和脂代谢中的分子机制.

主要方法:

病毒诱导的基因沉默 (VIGS) 降低小麦中的TaRBP1和TaGLTP表达.
对活性氧物种 (ROS) 积累和细胞死亡的分析.
蛋白质与蛋白质相互作用试验 (同聚合,TaRBP1-TaGLTP相互作用).
评估脂类代谢物水平和蛋白酶体依赖性降解.

主要成果:

通过增加ROS和细胞死亡,TaRBP1的淘汰增强了小麦对Pst的抵抗力,并将TaRBP1确定为负调节剂.
塔RBP1与TaGLTP (一种斯芬戈转移蛋白) 相互作用,TaGLTP的淘汰也使得Pst具有抗性.
TaRBP1通过抑制其26S蛋白酶体依赖性降解来稳定TaGLTP的积累,从而导致抑制ROS和脂积累.

结论:

揭示了一个新的易感机制,TaRBP1稳定了TaGLTP,在Pst感染期间抑制了植物防御.
这种相互作用通过调节脂代谢和ROS信号来微调小麦的防御反应.

关键词:

在Triticum aestivum中,它是最重要的.26S 蛋白质酶体是如何形成的?普奇尼亚形虫 (Puccinia striiformis f. sp. f. sp.) 是一种形虫. 这里是Triticity和Triticity的位置.植物免疫力植物免疫力有活性氧物种的反应性氧物种.球脂质是指脂质中的一种.

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