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Hsa_circ_0023826通过调节miR-188-3p/MDM4轴来保护对玻璃眼的保护

Bin Qu¹, Jing Wang¹, Yan Li¹

¹Department of Ophthalmology, Muping District Hospital of Traditional Chinese Medicine, Yantai City, Shandong Province, 253400, China.

Acta biochimica Polonica

|June 12, 2023

概括

循环RNAcirc_0023826通过调节miR-188-3p/MDM4轴来保护视网膜质细胞免受青光瘤的影响. 向circ_0023826为神经网膜退化提供了一个潜在的治疗策略.

科学领域:

分子生物学分子生物学
神经科学是一个神经科学.
眼科医生眼科眼科

背景情况:

循环RNAs (circRNAs) 参与神经系统疾病.
玻璃眼的特征是视网膜质细胞的损失.
青光眼病原体的精确机制需要进一步阐明.

研究的目的:

为了研究circ_0023826在青光眼引起的视网膜神经退行症中的作用.
探索涉及circ_0023826,miR-188-3p和小鼠双分钟4 (MDM4) 的调节轴.

主要方法:

在视网膜神经退行模型中分析circ_0023826表达.
在体内评估使用青光眼大鼠模型 (视觉行为,H&E染色).
在实验室中使用视网膜质细胞 (RGCs) (MTT,流细胞测量,西斑,ELISA),生物信息学,RNA下拉和光酶记者测试进行评估.

主要成果:

在视网膜神经退行症中,circ_0023826的表达被下调.
Upregulating circ_0023826 改善了老鼠的视觉功能和RGC在体外生存率.
Circ_0023826海绵式miR-188-3p,导致MDM4表达增加;MDM4沉默或miR-188-3p上调可以逆转这些效应.

结论:

通过调节miR-188-3p/MDM4通路,Circ_0023826对玻璃眼产生保护作用.
针对circ_0023826的目标上调是一种有前途的治疗方法,用于青光瘤和视网膜神经退行.

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