蛋白酶反应的凝微粒用于皮肤内药物输送
Heidi K Noddeland1,2, Marianne Lind2, Karsten Petersson2
1LEO Foundation Center for Cutaneous Drug Delivery, Department of Pharmacy, University of Copenhagen, 2100 Copenhagen, Denmark.
Biomacromolecules
|June 12, 2023
概括
新的蛋白酶反应微粒可以通过皮肤内递送药物. 这些对矩阵金属蛋白酶9 (MMP-9) 反应的水凝微粒使可调节的药物释放成为可能,对皮肤纤维细胞是安全的.
科学领域:
- 生物材料科学 生物材料科学
- 药物输送系统 药物输送系统
- 纳米技术纳米技术
背景情况:
- 皮肤内药物输送对于治疗皮肤疾病至关重要.
- 开发可注射,持续释放的药物库是一个关键的挑战.
- 蛋白酶响应材料提供了针对药物释放的潜力.
研究的目的:
- 开发用于皮肤内药物输送的蛋白酶反应微粒.
- 评估矩阵金属蛋白酶9 (MMP-9) 对微粒物质和药物释放的影响.
- 评估微粒子特征的可调性及其安全性.
主要方法:
- 逆悬浮光聚合制造以创建多臂聚乙烯糖醇基微粒.
- 使用扫描电子显微镜和原子力显微镜进行表征.
- 使用托法西提尼布酸盐的体外释放研究和使用皮肤纤维细胞的细胞毒性测定.
主要成果:
- 球形微粒 (∼40μm) 适用于皮内注射 (27G针).
- 暴露于MMP-9导致网络退化和弹性模块的减少.
- 多次MMP-9暴露显著增加了facitinib酸盐从响应的微粒中释放的量.
- 微粒子特性 (释放特征,弹性) 可以通过改变聚乙烯糖醇复杂度来调整.
- 细胞毒性研究没有显示对皮肤纤维细胞的不良影响.
结论:
- 蛋白酶响应的微粒体显示出控制的皮肤内药物递送的显著潜力.
- 开发的微粒提供可调节的药物释放配置和机械性能.
- 这些微粒对于皮肤细胞的使用是安全的,支持它们的治疗应用.
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