一个双门结构无损离子操纵-离子移动轨道轨道轨道质谱平台的双门结构,用于组合超高分辨率分子分析
Adam L Hollerbach1, Yehia M Ibrahim1, Vanessa Meras1
1Biological Sciences Division, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99354, United States.
Analytical chemistry
|June 12, 2023
概括
我们开发了一个新的高分辨率离子移动性光谱-质谱 (HR-IMS-MS) 平台,通过将无损离子操纵结构 (SLIM) 与轨道飞行器MS结合起来. 这种集成系统可以同时进行分离和质量分析,以提高生物混合物的特性.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 物理化学 物理化学
背景情况:
- 高分辨率离子移动性光谱-质谱 (HR-IMS-MS) 对于分析复杂的生物样本至关重要.
- 目前的HR-IMS和HR-MS分析通常由于时间尺度不匹配而单独执行.
- 需要采用统一的方法来加强生物混合物的表征.
研究的目的:
- 开发和验证一个新的平台,将HR-IMS与HR-MS集成,用于同时分析.
- 克服独立HR-IMS和HR-MS测量的时间限制.
- 为了使复杂的生物样本,包括蛋白质组学和脂质组学,能够进行全面的表征.
主要方法:
- 通过使用双门离子注射系统将一个11米结构无损离子操纵 (SLIM) 模块合到Q-Exactive Plus Orbitrap MS.
- 实现同时进行SLIM分离,高分辨率轨道飞行器质量分析 (高达140k),以及高能碰撞诱导解离 (HCD).
- 使用酸,标准和复杂的脂质混合物来表征平台.
主要成果:
- 在大约25分钟内,SLIM-Orbitrap平台实现了同时进行HR-IMS-MS/MS分析.
- 显示了平均SLIM碰撞横截面 (CCS) 解析功率 (RpCCS) 约218和峰值容量约156.
- 成功分离异脂和识别,展示了该系统对复杂混合物分析的实用性.
结论:
- 开发的双门SLIM-Orbitrap平台可以实现集成的高分辨率离子移动性和质谱.
- 这种新的功能通过提供高分辨率的多式联络数据,显著推进了蛋白质组学和脂组学.
- 该系统为在复杂的生物样本中识别未知离子结构的无参考识别奠定了基础.
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