Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.1K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.4K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Antifungal Effects of Terminalia arjuna Fruit Extract on Multidrug-Resistant Candida auris: An In Vitro Study.

Cureus·2026
Same author

Onychomycosis caused by <i>Chaetomium globosum</i>: an uncommon fungal nail infection.

Current medical mycology·2026
Same author

Conserved post-odor dynamics in the olfactory systems of mice and locusts.

iScience·2026
Same author

Assessment of sensitivity of low-cost fluorescein as an alternative to radiocolloid for sentinel lymph node biopsy in breast carcinoma.

Reports of practical oncology and radiotherapy : journal of Greatpoland Cancer Center in Poznan and Polish Society of Radiation Oncology·2026
Same author

Maintenance of oral hygiene with beetroot rinse in gingivitis patients with oral lichen planus: A pilot randomized controlled trial.

Journal of Indian Society of Periodontology·2026
Same author

Rapid urinary lipoarabinomannan test with laboratory-level sensitivity for tuberculosis detection: a performance evaluation.

Microbiology spectrum·2025

相关实验视频

Updated: Jul 26, 2025

Author Spotlight: Universal Molecular Retention with 11-Fold Expansion Microscopy
10:31

Author Spotlight: Universal Molecular Retention with 11-Fold Expansion Microscopy

Published on: October 6, 2023

7.5K

增强等离子体扩展显微镜

Priya Rathi1, Prashant Gupta2, Avishek Debnath2

  • 1Department of Chemistry, Washington University in St. Louis, St. Louis, Missouri 63130, United States.

Nano letters
|June 12, 2023
PubMed
概括

增强等离子体扩展显微镜 (p-ExM) 使用明亮的等离子体纳米标签来克服扩展显微镜 (ExM) 中的信号稀释. 这种新的技术显著改善了神经网络追踪和形态分析.

关键词:
扩张显微镜扩张显微镜光显微镜的光显微镜.nanoneuro 相互作用 相互作用用等离子体增强的光.塑-的使用方法

更多相关视频

Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy
08:16

Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy

Published on: February 7, 2025

525
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.1K

相关实验视频

Last Updated: Jul 26, 2025

Author Spotlight: Universal Molecular Retention with 11-Fold Expansion Microscopy
10:31

Author Spotlight: Universal Molecular Retention with 11-Fold Expansion Microscopy

Published on: October 6, 2023

7.5K
Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy
08:16

Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy

Published on: February 7, 2025

525
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.1K

科学领域:

  • 生物物理学的生物物理.
  • 显微镜的使用方法
  • 纳米技术纳米技术

背景情况:

  • 扩展显微镜 (ExM) 通过同位素扩展的生物样本来提高空间分辨率.
  • ExM的一个主要限制是光信号稀释,阻碍了其广泛采用.
  • 需要改进的纳米标签来增强ExM中的信号强度和保留.

研究的目的:

  • 引入增强等离子体膨胀显微镜 (p-ExM) 以克服ExM中的信号稀释.
  • 为了利用超明亮的等离子体 (PF) 纳米结构作为p-ExM的纳米标签.
  • 为了证明p-ExM在改善神经网络分析方面的有效性.

主要方法:

  • 塑 (PF) 纳米标签的开发和应用.
  • 实施生物样本扩展的p-ExM协议.
  • 使用p-ExM进行神经网络形态的成像和量化.

主要成果:

  • 与传统标签 (<16%) 相比,PFs具有显著更高的光强度 (15000倍) 和保留率 (76%).
  • p-ExM可以改进神经网络的追踪和解密.
  • 对形态标记物的量化显示神经元末端点增加了2.5倍.

结论:

  • p-ExM有效地解决了ExM中的信号稀释挑战.
  • PF纳米标签在ExM中的超分辨率成像中非常有效.
  • p-ExM为研究生物系统中的结构功能关系提供了有价值的工具.