相关实验视频

Updated: Jul 26, 2025

Multiplexed Fluorescent Immunohistochemical Staining of Four Endometrial Immune Cell Types in Recurrent Miscarriage

Multiplexed Fluorescent Immunohistochemical Staining of Four Endometrial Immune Cell Types in Recurrent Miscarriage

Published on: August 4, 2021

热原体PR-SET7功能障碍诱导病毒模仿反应和与反复流产相关的亡

Xiaobo Zhou^1,2, Yingchun Xu¹, Shengnan Ren¹

¹Fujian Provincial Key Laboratory of Reproductive Health Research, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, Fujian 361102, China.

Proceedings of the National Academy of Sciences of the United States of America

|June 12, 2023

概括

酶PR-SET7对于抑制质细胞内源性逆转录病毒至关重要,防止妊娠流产. 它的失调与反复流产有关,这表明表观遗传因素有助于这种情况.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
生殖生物学生殖生物学
分子遗传学分子遗传学

背景情况:

复发性流产 (RM) 是一种怀孕并发症,原因不明.
热囊细胞功能障碍越来越多地与RM病变产生有关.
调节H4K20单甲基化的一种酶PR-SET7在热囊细胞和RM中的作用尚不清楚.

研究的目的:

为了研究PR-SET7在热囊细胞中的功能.
为了确定PR-SET7与反复流产的相关性.

主要方法:

在小鼠中,P-set7的热囊细胞特异性淘汰.
对内源性逆转录病毒 (ERVs) 的分析.
评估干扰素反应和亡.
对H4K20甲基化状态的表观遗传学分析.
在人体RM胎盘中检查PR-SET7表达.

主要成果:

在小鼠的热囊细胞中,PR-SET7的损失导致了缺陷的热囊细胞和早期的胚胎损失.
由于PR-SET7的缺乏,导致ERVs的脱压,病毒模仿和干扰素驱动的亡.
H4K20me1和H4K20me3被确定为ERV抑制的媒介.
在RM胎盘中发现了异常的PR-SET7表达和表观遗传变化.

结论:

通过H4K20甲基化,PR-SET7对于通过H4K20甲基化抑制 trofoblasts中的ERVs至关重要.
PR-SET7功能障碍有助于 trofhoblast 缺陷和胚胎损失.
PR-SET7的表观遗传调节失调为反复流产的病因提供了新的洞察力.

关键词:

在ERV系统中,H4K20me1/3 的时间这就是PR-SET7的原因.经常发生的流产.病毒模仿病毒模仿

更多相关视频

siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts

siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts

Published on: September 20, 2016

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

Published on: March 16, 2017

相关概念视频

Teratogenicity

Teratogenicity

The ability of a drug to produce structural deformations and functional abnormalities in the developing embryo or the fetus is called teratogenicity, and the drug producing this effect is known as a teratogen. Teratogenic effects include stillbirth, miscarriage, intrauterine growth restriction, and neurocognitive delay. A teratogen may affect the embryo at different stages of development, which is important in determining the type and extent of the damage. During blastocyst formation, the early...