复制子:是编码HUH内核酶的真核转子的一个主要组
1California Institute for Quantitative Biosciences, University of California, Berkeley, CA 94720.
概括
研究人员发现了Replitrons,一种新型的真核转子体组. 这些转位子,编码Rep HUH内核酶,具有古老和独立的起源,扩大已知的移动基因元素在真核细胞的多样性.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 进化生物学 进化生物学
背景情况:
- 复制 (复制蛋白) 类的HUH内核酶对于复制各种病毒基因组和等离子体至关重要.
- HUH转基因酶从Reps进化,形成了主要的可转基因元素组,如IS200/IS605,IS91/ISCR和真核生物的海立子.
研究的目的:
- 确定和描述一种新型的真核转子组,称为Replitrons,编码Rep HUH内核酶.
- 调查Replitrons与其他含有HUH内核酶的元素的进化起源和关系.
主要方法:
- 生物信息分析包括蛋白质聚类以确定进化关系.
- 预测Replitron转换酶的三级结构.
- 长读序列测定,以描述Replitron-1在*Chlamydomonas reinhardtii*中的新插入.
主要成果:
- 反复子代表了一组独特的真核转子子,在它们的Rep域结构中与Helitrons不同.
- 反复子转体酶与来自循环反复编码单链 (CRESS) DNA 病毒和质粒 (pCRESS) 的 Reps 的弱相关性.
- 复制子存在于多个真核超群中,在非种子植物中达到很高的副本数量,并表现出复制粘贴插入机制.
结论:
- 复原体具有古老且在进化上独立的起源,与已知的HUH转基因酶分开.
- 这一发现显著扩大了已知的转子体和HUH内核酶在真核生物中的多样性.
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